Description

5-Methyl-[2'-13C]Uridine is a stable isotope-labeled nucleoside analog, specifically labeled with carbon-13 at the 2' position of the ribose ring. This precise labeling makes it an essential tool for advanced metabolic and pharmacokinetic studies, providing critical insights into RNA synthesis, modification, and turnover pathways. It is primarily used by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for applications in drug development, tracer studies, and analytical method validation.

Application

• Metabolic Tracer Studies: Used as a stable isotope-labeled precursor to track and quantify the synthesis and catabolism of RNA in cell culture and in vivo models.
• Drug Metabolism & Pharmacokinetics (DMPK): Serves as an internal standard in LC-MS/MS and GC-MS analyses for the accurate quantification of nucleoside-based drugs and their metabolites.
• Biochemical Research: Employed in enzymatic studies to investigate the mechanisms and kinetics of RNA-modifying enzymes, such as methyltransferases.
• Diagnostic Assay Development: Utilized in the calibration and validation of mass spectrometry-based diagnostic tests for detecting abnormal RNA modifications linked to disease.
• Isotope Dilution Mass Spectrometry (IDMS): Acts as a high-purity reference standard to ensure accuracy and precision in quantitative analytical methods.

Basic Information

Product Name	5-Methyl-[2'-13C]Uridine
CAS No.	478510-98-4
Molecular Formula	C10H1413CN2O6
Molecular Weight	260.23 g/mol (for 13C labeled form)
Synonyms	5-Methyluridine-2'-13C; 2'-Deoxy-5-methyl-[2'-13C]uridine; [2'-13C]-5-Methyluridine; 5-Methyl-2'-deoxy[2'-13C]uridine; m5U-[2'-13C]; Stable Isotope Labeled Uridine Derivative; RNA Modification Tracer
EINECS	Contact for details

Quality Control

Every batch of 5-Methyl-[2'-13C]Uridine is produced and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, verified by advanced spectroscopic and chromatographic techniques including NMR, HPLC, and MS. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic enrichment, and analytical results is provided with each shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or with desiccant to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 99 atom % 13C
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.