Description

5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxy-3-Formylindole-Ribofuranosyl-3'-[(2-Cyano Ethyl)-(N,N-Diisopropyl)]-Phosphoramidite is a highly specialized, protected nucleoside phosphoramidite monomer used in automated solid-phase oligonucleotide synthesis. This compound is critical for the precise and efficient incorporation of modified nucleosides into synthetic DNA and RNA strands, enabling the creation of advanced oligonucleotides with tailored properties. It is an essential building block for research and development in pharmaceutical discovery, molecular diagnostics, and biotechnology, particularly for applications requiring non-standard bases.

Application

• Oligonucleotide Synthesis: Primary monomer for the automated solid-phase synthesis of DNA strands containing modified indole nucleoside analogs.
• Antisense & siRNA Therapeutics: Key raw material in the production of therapeutic oligonucleotides designed for gene silencing and targeted drug development.
• Molecular Probes & Diagnostics: Used to manufacture fluorescently labeled or otherwise tagged probes for PCR, sequencing, and hybridization assays.
• Aptamer Development: Enables the synthesis of SELEX libraries and specific aptamers with enhanced binding characteristics through base modification.
• Structural Biology Studies: Facilitates the creation of oligonucleotides with site-specific modifications for NMR and X-ray crystallography studies.
• Synthetic Biology: Building block for constructing artificial genes and genetic circuits with novel functionalities.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxy-3-Formylindole-Ribofuranosyl-3'-[(2-Cyano Ethyl)-(N,N-Diisopropyl)]-Phosphoramidite
CAS No.	460355-05-9
Molecular Formula	C49H55N4O8P
Molecular Weight	859.0 g/mol
Synonyms	3-Formylindole dC Phosphoramidite; 5'-O-DMT-2'-Deoxy-3-formylindole-riboside-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dCFInd Phosphoramidite; 2'-Deoxy-3-formylindole-5'-O-DMT-3'-phosphoramidite; N4-[(Dimethoxytrityl)-2'-deoxy-3-formylindole-ribosyl]-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Modified Nucleoside Phosphoramidite; CAS 460355-05-9
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is manufactured and tested under strict quality control protocols to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Our standard HPLC purity specification exceeds 98%, with stringent controls for moisture content and reactive impurities. A comprehensive Certificate of Analysis (COA) detailing purity (HPLC), identity (NMR, MS), and moisture content (Karl Fischer) is provided with each shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 98.0%
Moisture Content (Karl Fischer)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.