Description

N4-Benzoyl-5'-O-(4,4'-Dimethoxytrityl)-5-Methyl-2'-O,4'-C-Methylenecytidine is a sophisticated, high-purity nucleoside phosphoramidite derivative, a critical building block in oligonucleotide synthesis. Its value lies in providing precise chemical control for the incorporation of modified cytidine analogs into synthetic DNA and RNA strands, enabling advanced research and therapeutic development. This compound is essential for pharmaceutical R&D, biotechnology, and academic laboratories focused on antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based technologies. The product is supplied under stringent quality control to ensure optimal performance in automated solid-phase synthesis.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of modified DNA/RNA strands.
• Antisense Drug Development: For creating therapeutic oligonucleotides targeting specific mRNA sequences.
• siRNA and miRNA Research: Enables the production of chemically stabilized small interfering RNA and microRNA analogs with enhanced nuclease resistance.
• Aptamer Production: Used in synthesizing libraries of nucleic acid aptamers for diagnostic and therapeutic targeting.
• Structural Biology Studies: Incorporation into oligonucleotides to study nucleic acid structure, stability, and protein interactions.
• Diagnostic Probe Development: For creating high-affinity, labeled probes used in PCR, FISH, and other molecular diagnostic techniques.

Basic Information

Item	Detail
Product Name	N4-Benzoyl-5'-O-(4,4'-Dimethoxytrityl)-5-Methyl-2'-O,4'-C-Methylenecytidine
CAS No.	445012-77-1
Molecular Formula	C48H45N3O8
Molecular Weight	791.90 g/mol
Synonyms	5-Methyl-2'-O,4'-C-methylenecytidine, N4-benzoyl-5'-O-(4,4'-dimethoxytrityl)-; 5'-O-DMT-5-methyl-2'-O,4'-C-methylenecytidine, N4-benzoyl-; LNA-C(Bz) Phosphoramidite precursor; 5-Methyl-2',4'-BNA/LNA-C(Bz) DMT; Bn-C ENA amidite (protected); Constrained Ethyl Nucleoside Analog; Bicyclic Cytidine Derivative; Locked Nucleic Acid Cytidine Monomer (protected)
EINECS	Contact for details

Quality Control

Every batch of N4-Benzoyl-5'-O-(4,4'-Dimethoxytrityl)-5-Methyl-2'-O,4'-C-Methylenecytidine is manufactured and tested under a quality management system. The product undergoes rigorous analytical characterization to ensure it meets the high-purity standards required for sensitive oligonucleotide synthesis. Standard testing includes HPLC for purity and identity confirmation (typically ≥98%), 1H/13C NMR for structural verification, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, dichloromethane, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.