Description

1-(2'-Deoxy-3'-o-(4-Nitrobenzenesulfonyl)-5'-o-(4,4'-Dimethoxytrityl)-β-D-Threo-Pentafuranosyl)-3-(tert-Butyloxycarbonyl)Thymine is a sophisticated, high-purity nucleoside phosphoramidite derivative, a critical building block in the automated synthesis of oligonucleotides. Its value lies in enabling the precise and scalable production of complex DNA and RNA sequences for advanced research and therapeutic development. This compound is essential for pharmaceutical R&D teams, diagnostic developers, and academic laboratories working on antisense oligonucleotides, siRNA, aptamers, and other nucleic acid-based technologies.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated, stepwise assembly of custom DNA and RNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and other gene-silencing therapies.
• Diagnostic Probe Development: Synthesis of labeled probes for PCR, microarrays, and fluorescence in situ hybridization (FISH).
• Research-Grade Oligo Synthesis: For academic and industrial R&D requiring high-fidelity, modified oligonucleotides.
• Aptamer and SELEX Library Construction: Building blocks for creating vast libraries of nucleic acid aptamers.
• Synthesis of Modified Nucleosides: Intermediate for further chemical modification to create novel nucleoside analogs.

Basic Information

Product Name	1-(2'-Deoxy-3'-o-(4-Nitrobenzenesulfonyl)-5'-o-(4,4'-Dimethoxytrityl)-β-D-Threo-Pentafuranosyl)-3-(tert-Butyloxycarbonyl)Thymine
CAS No.	444717-23-1
Molecular Formula	C46H47N5O13S
Molecular Weight	910.0 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-3'-O-(4-nitrobenzenesulfonyl)thymidine 3'-(2-Cyanoethyl N,N-diisopropylphosphoramidite); dT(Nosyl) Phosphoramidite (protected); 3'-O-(4-Nitrobenzenesulfonyl)-5'-O-(4,4'-dimethoxytrityl)thymidine 3'-(2-cyanoethyl N,N-diisopropylphosphoramidite); Thymidine, 2'-deoxy-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-3'-O-[(4-nitrophenyl)sulfonyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; N-T-BOC-5'-O-DMT-2'-deoxy-3'-O-(4-nitrobenzenesulfonyl)thymidine; BOC-dT(Nosyl) Phosphoramidite
EINECS	Contact for details

Quality Control

This high-value synthetic intermediate is produced under strict quality control protocols in a GMP-compliant environment. Every batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure structural integrity and purity. A comprehensive Certificate of Analysis (COA) detailing identity, assay, purity, and moisture content is provided with each shipment to support your regulatory and R&D documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Assay	≥ 95.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.