Description

1-[5-o-(4,4'-Dimethoxytrityl)-3-o-Nitrophenylsulfonyl-2-Deoxy-β-D-Lyxofuranosyl]Thymine is a sophisticated nucleoside derivative, specifically a protected thymidine analog, engineered for advanced oligonucleotide synthesis. This compound is a critical high-purity building block in the production of antisense oligonucleotides, primers, and probes for therapeutic and diagnostic applications. It is essential for research institutions and pharmaceutical manufacturers engaged in cutting-edge genetic medicine and molecular biology.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A key phosphoramidite precursor for the automated synthesis of DNA and RNA sequences.
• Antisense Oligonucleotide (ASO) Development: Used in the research and manufacturing of therapeutic oligonucleotides for targeting specific RNA sequences.
• Primer and Probe Synthesis: Essential for creating high-fidelity primers for PCR and labeled probes for diagnostic assays and genomic research.
• siRNA and miRNA Synthesis: Serves as a protected monomer in the construction of small interfering RNA (siRNA) and microRNA (miRNA) strands.
• Pharmaceutical Intermediate: A crucial raw material in the cGMP-compliant production of nucleic acid-based active pharmaceutical ingredients (APIs).
• Academic and Industrial R&D: Used in laboratories for studying nucleic acid chemistry, developing novel synthetic routes, and creating custom oligonucleotide libraries.

Basic Information

Product Name	1-[5-o-(4,4'-Dimethoxytrityl)-3-o-Nitrophenylsulfonyl-2-Deoxy-β-D-Lyxofuranosyl]Thymine
CAS No.	444717-20-8
Molecular Formula	C41H39N3O11S
Molecular Weight	781.83 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-3'-O-(2-nitrophenylsulfonyl)-2'-deoxy-β-D-lyxofuranosyl thymine; 3'-O-(2-Nitrophenylsulfonyl)-5'-O-(4,4'-dimethoxytrityl)thymidine; 3'-O-Nosyl-5'-O-DMTr-thymidine (lyxo isomer); DMTr-dT(Lyxo)-3'-ONs; Thymidine, 3'-(2-nitrophenylsulfonyl)-5'-(4,4'-dimethoxytrityl)-, 2'-deoxy-β-D-lyxofuranosyl derivative; Protected Thymidine Lyxofuranosyl Derivative; Oligonucleotide Synthesis Building Block 444717-20-8
EINECS	Contact for details

Quality Control

Our production of this high-value nucleoside derivative adheres to strict quality management systems, ensuring batch-to-batch consistency for sensitive oligonucleotide synthesis. Quality is verified through comprehensive analytical methods including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and suitability for phosphoramidite chemistry. We support development under GMP-grade conditions upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.