Description

Sulphosuccinimidyl 4-(4-Azidophenyl)Butyrate is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a photoactivatable phenyl azide group. This compound is essential for creating stable covalent bonds between biomolecules under mild, controlled conditions, enabling the study of protein interactions, cell surface labeling, and the development of diagnostic assays. It is a critical tool for researchers and developers in bioconjugation, proteomics, pharmaceutical development, and materials science who require precise and efficient crosslinking strategies.

Application

• Bioconjugation and Protein Crosslinking: For covalently linking proteins, peptides, or antibodies to other molecules, surfaces, or solid supports.
• Photoaffinity Labeling: Used to capture transient or weak biological interactions by crosslinking binding partners upon UV light activation.
• Surface Immobilization: Functionalizing chips, beads, or biosensor surfaces with biomolecules for assay development and diagnostic applications.
• Proteomics Research: Identifying protein-protein interactions and mapping protein networks within complex biological samples.
• Drug Delivery System Development: Conjugating therapeutic agents to carriers or targeting moieties.
• Polymer and Biomaterial Modification: Introducing bioactive groups into synthetic polymers or hydrogels to create functional biomaterials.

Basic Information

Product Name	Sulphosuccinimidyl 4-(4-Azidophenyl)Butyrate
CAS No.	441052-82-0
Molecular Formula	C18H19N3O6S
Molecular Weight	405.43 g/mol
Synonyms	Sulfo-SANPAH; N-Sulfosuccinimidyl 4-Azidobenzoate (common misnomer); 4-(4-Azidophenyl)butyric acid sulfo-NHS ester; Sulfo-NHS-APB; Heterobifunctional crosslinker, NHS-ester/Phenyl Azide; 4-Azidophenylbutyrate NHS ester, sulfonated; N-Hydroxysulfosuccinimide ester of 4-(4-azidophenyl)butyric acid
EINECS	Contact for details

Quality Control

Our Sulphosuccinimidyl 4-(4-Azidophenyl)Butyrate is manufactured under strict quality protocols to ensure high purity and consistent performance for sensitive research applications. Each batch is analyzed to confirm critical parameters such as purity, functional group activity, and the absence of interfering impurities. Certificates of Analysis (COA) with detailed HPLC, NMR, and MS data are provided to guarantee traceability and support your research integrity.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. The product is light-sensitive (store away from light) and moisture-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content	≤ 1.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.