Description

N3-Peg6-Tbu CAS NO 406213-76-1 is a heterobifunctional polyethylene glycol (PEG) linker featuring an azide (N3) group and a tert-butyl (tBu) protected terminal, connected by a hexaethylene glycol (PEG6) spacer. This compound is a critical building block in bioconjugation and click chemistry, enabling the precise, high-yield attachment of biomolecules, drugs, or probes under mild, aqueous conditions. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostics industries for applications such as ADC development, protein modification, and surface functionalization.

Application

• Antibody-Drug Conjugate (ADC) Linker: Serves as a key component for constructing stable, cleavable linkers between cytotoxic payloads and monoclonal antibodies.
• PEGylation Reagent: Used to modify peptides, proteins, and oligonucleotides to enhance solubility, stability, and pharmacokinetic profiles.
• Click Chemistry for Bioconjugation: The azide group readily participates in copper-catalyzed or strain-promoted azide-alkyne cycloaddition (SPAAC) with alkynes for site-specific labeling.
• Surface Functionalization: Enables the modification of nanoparticles, microarrays, and biosensor surfaces with bioactive molecules.
• Polymer and Materials Science: Acts as a versatile monomer or crosslinker for synthesizing functional hydrogels and smart biomaterials.
• Diagnostic Probe Synthesis: Facilitates the creation of imaging agents and fluorescent probes for in vitro and in vivo diagnostics.

Basic Information

Product Name	N3-Peg6-Tbu
CAS No.	406213-76-1
Molecular Formula	C25H49N3O8
Molecular Weight	519.68 g/mol
Synonyms	Azido-PEG6-t-butyl ester; 1-Azido-3,6,9,12,15,18-hexaoxahenicosan-21-oic acid tert-butyl ester; N3-PEG6-OtBu; tert-Butyl 22-azido-4,7,10,13,16,19-hexaoxadocosanoate; Azido-PEG6-acid t-butyl ester; PEG6 azide t-butyl ester; Heterobifunctional PEG linker N3-PEG6-tBu
EINECS	Contact for details

Quality Control

Our N3-Peg6-Tbu is manufactured under strict quality management systems. Every batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and batch-to-batch consistency suitable for research and cGMP manufacturing. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.