Description

N4-Benzoyl-5'-o-(4,4-Dimethoxytrityl)-2'-Deoxycytidine-3'-Succinate, Tea Salt is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in automated oligonucleotide synthesis. This compound is essential for ensuring high coupling efficiency and sequence fidelity in the production of DNA and RNA strands. It is primarily required by pharmaceutical R&D teams, diagnostic kit manufacturers, and biotechnology companies engaged in the development of antisense oligonucleotides, primers, probes, and therapeutic nucleic acids.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of DNA sequences, particularly at cytidine positions.
• Therapeutic Nucleic Acid Development: For the research and GMP-scale production of antisense oligonucleotides (ASOs), siRNA, and aptamers for targeted therapies.
• Diagnostic Probe Manufacturing: Used in the synthesis of labeled probes and primers for PCR, qPCR, and next-generation sequencing (NGS) applications.
• Research & Development: A vital reagent in academic and industrial labs for studying gene function, gene editing (e.g., CRISPR guide RNA synthesis), and molecular biology.
• Scale-Up cGMP Production: Suitable for scaling up to meet the stringent quality requirements for clinical-grade oligonucleotide active pharmaceutical ingredients (APIs).

Basic Information

Product Name	N4-Benzoyl-5'-o-(4,4-Dimethoxytrityl)-2'-Deoxycytidine-3'-Succinate, Tea Salt
CAS No.	402944-20-1
Molecular Formula	C48H45N3O10
Molecular Weight	823.89 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-benzoyl-2'-deoxycytidine-3'-succinyl TEA salt; dC(Bz) Phosphoramidite Synthon; N4-Benzoyl-5'-O-DMT-2'-deoxycytidine-3'-succinic acid TEA salt; dC(Bz)-CE Phosphoramidite precursor; Protected deoxycytidine-3'-succinate; 2'-Deoxycytidine, N4-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-(hydrogen succinate), compd. with triethylamine (1:1); CAS 402944-20-1
EINECS	Contact for details

Quality Control

Our N4-Benzoyl-5'-o-(4,4-Dimethoxytrityl)-2'-Deoxycytidine-3'-Succinate, Tea Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (typically ≥98.5%), to ensure it meets the exacting standards for oligonucleotide synthesis. Certificates of Analysis (COA) with full chromatographic data are provided, supporting compliance with research and cGMP guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed bag with argon or nitrogen atmosphere) due to its hygroscopic and moisture-sensitive nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.