Description

N6-Benzoyl-5'-O-(4,4-Dimethoxytrityl)-2'-Deoxyadenosine-3'-Succinate, Tea Salt is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in automated oligonucleotide synthesis. This compound is essential for the precise and efficient assembly of DNA sequences, ensuring high coupling efficiency and yield. It is primarily required by biotechnology, pharmaceutical, and diagnostic companies engaged in the research and production of antisense oligonucleotides, primers, probes, and therapeutic nucleic acids.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA strands.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense drugs, siRNA, and aptamers for targeted therapies.
• Diagnostic Probe Development: Synthesis of labeled probes for PCR, qPCR, FISH, and microarray applications.
• Research & Development: Used in academic and industrial labs for custom gene synthesis and molecular biology research.
• Next-Generation Sequencing (NGS): Manufacture of primers and adapters for sequencing platforms.
• CRISPR/Cas9 System Components: Synthesis of guide RNAs (gRNAs) and other synthetic nucleic acid components.

Basic Information

Product Name	N6-Benzoyl-5'-O-(4,4-Dimethoxytrityl)-2'-Deoxyadenosine-3'-Succinate, Tea Salt
CAS No.	402944-15-4
Molecular Formula	C48H45N5O10
Molecular Weight	851.91 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-benzoyl-2'-deoxyadenosine-3'-succinate, TEA Salt; dA(Bz) Phosphoramidite Synthon; N6-Benzoyl-2'-deoxyadenosine-3'-succinyl TEA salt, DMT-protected; 2'-Deoxyadenosine, N6-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-(hydrogen succinate), compd. with N-ethylethanamine (1:1); Protected Deoxyadenosine Phosphoramidite Intermediate; dA-Bz-CE Phosphoramidite precursor
EINECS	Contact for details

Quality Control

This high-value synthetic intermediate is produced under strict quality control in a GMP-compliant environment. Every batch is characterized by advanced analytical techniques including HPLC, NMR, and Mass Spectrometry to ensure identity, purity, and suitability for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided, confirming compliance with stringent in-house specifications for critical parameters like moisture content and reactive impurities.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility Test	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.