Description

3',5'-o-Dtbs-2'-o-Tbdms-N4-Ac-Rc CAS NO 401812-96-2 is a high-purity, protected nucleoside phosphoramidite derivative, a critical building block in the automated synthesis of oligonucleotides. Its value lies in providing precise control over nucleic acid sequence assembly, ensuring high coupling efficiency and yield for demanding research and therapeutic applications. This compound is essential for biotechnology and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNAs, aptamers, and other advanced genetic medicines.

Application

• Oligonucleotide Synthesis: Serves as a key phosphoramidite monomer in solid-phase automated DNA/RNA synthesizers.
• Therapeutic Oligo Development: Critical for the research and GMP-scale production of antisense, siRNA, and miRNA-based therapeutics.
• Diagnostic Probe Manufacture: Used in the synthesis of labeled probes for PCR, FISH, and microarray technologies.
• Academic & Industrial Research: Enables basic research in molecular biology, genetics, and drug discovery programs.
• Aptamer Production: Facilitates the synthesis of complex, modified aptamers for sensing and targeting applications.

Basic Information

Product Name	3',5'-o-Dtbs-2'-o-Tbdms-N4-Ac-Rc
CAS No.	401812-96-2
Molecular Formula	C33H55N4O7PSi2
Molecular Weight	707.02 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N4-acetyl-cytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; N4-Acetyl-5'-O-DMT-2'-O-TBDMS-cytidine-3'-CE phosphoramidite; dC(Ac) Amidite, 2'-O-TBDMS protected; rC(Ac) Phosphoramidite; Cytidine, 2'-O-(1,1-dimethylethyl)dimethylsilyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N-acetyl-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; 401812-96-2; 2'-O-TBDMS-N4-Ac-CE Phosphoramidite
EINECS	Contact for details

Quality Control

Our 3',5'-o-Dtbs-2'-o-Tbdms-N4-Ac-Rc is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for critical synthesis workflows. Each lot undergoes comprehensive analytical testing, including HPLC purity assay, 31P NMR, and MS confirmation, to meet stringent specifications for oligonucleotide synthesis. Certificates of Analysis (COA) with full traceability are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C ± 5°C under an inert atmosphere (argon or nitrogen). This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. Use in a dry, controlled environment.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture (KF)	≤ 1.0%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.