Description

4-Azidobenzoic Acid n-Hydroxy-Sulfosuccinimide Ester Sodium Salt is a specialized, water-soluble heterobifunctional crosslinking reagent designed for bioconjugation. This compound matters because it enables the efficient and controlled coupling of biomolecules, such as proteins and peptides, to other surfaces or molecules under mild aqueous conditions. It is primarily needed by researchers and manufacturers in the fields of biopharmaceutical development, diagnostic assay production, and advanced materials science for creating stable, functionalized conjugates.

Application

• Bioconjugation and Crosslinking: A key reagent for covalently linking proteins, antibodies, or peptides to polymers, nanoparticles, or solid surfaces via its amine-reactive NHS ester and photoactivatable azide groups.
• Diagnostic Kit Manufacturing: Used in the development of immunoassays (e.g., ELISA) and lateral flow tests to immobilize capture antibodies or antigens onto membranes or microplates.
• Drug Delivery System Development: Facilitates the surface functionalization of liposomes, dendrimers, or polymeric nanoparticles for targeted therapeutic delivery.
• Biomaterial and Hydrogel Fabrication: Enables the photo-initiated crosslinking of polymers to create scaffolds for tissue engineering and 3D cell culture.
• Affinity Chromatography Resin Preparation: Used to couple specific ligands to chromatographic supports for protein purification.
• Proteomics and Chemical Biology Research: Serves as a tool for labeling, pull-down assays, and studying protein-protein interactions.

Basic Information

Product Name	4-Azidobenzoic Acid n-Hydroxy-Sulfosuccinimide Ester Sodium Salt
CAS No.	401572-86-9
Molecular Formula	C11H7N4NaO7S
Molecular Weight	362.25 g/mol
Synonyms	Sulfo-SANPAH; 4-Azidobenzoic Acid, 2,5-dioxo-1-pyrrolidinyl ester, sodium salt, sulfonated; Sulfo-Succinimidyl 4-Azidobenzoate; Sodium 4-((2,5-dioxopyrrolidin-1-yl)oxy)-4-oxo-3-sulfonatobenzeneazidate; N-Sulfosuccinimidyl 4-Azidobenzoate; NHS-Azide, sulfonated; Water-soluble NHS-Azide; Heterobifunctional Crosslinker, Sulfo-SANPAH
EINECS	Contact for details

Quality Control

Our 4-Azidobenzoic Acid n-Hydroxy-Sulfosuccinimide Ester Sodium Salt is manufactured under strict quality protocols to ensure batch-to-batch consistency and performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and functional group verification. Certificates of Analysis (COA) detailing identity, purity, and assay are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Freely soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.