Description

5'-o-tert-Butyldimethylsilyl-Thymidine is a key protected nucleoside derivative, essential for the controlled synthesis of oligonucleotides and nucleic acid analogs. This compound provides a critical hydroxyl-protecting group strategy, enabling precise and high-yield chemical transformations in complex molecular assembly. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of novel therapeutics, diagnostic probes, and advanced genetic research materials.

Application

• Oligonucleotide Synthesis: Serves as a vital building block (phosphoramidite precursor) in the solid-phase synthesis of DNA and RNA strands for research and therapeutic applications.
• Antiviral & Anticancer Prodrug Development: Used as an intermediate in the synthesis of nucleoside-based prodrugs and active pharmaceutical ingredients (APIs).
• Chemical Biology Research: Employed in the study of nucleic acid structure, function, and for creating labeled or modified nucleotides for probes.
• Protecting Group Chemistry: The tert-butyldimethylsilyl (TBDMS) group at the 5'-position allows for selective deprotection in multi-step synthetic routes.
• Synthesis of Nucleoside Analogs: A crucial starting material for creating modified nucleosides with altered biological activity or stability.

Basic Information

Product Name	5'-o-tert-Butyldimethylsilyl-Thymidine
CAS No.	40733-28-6
Molecular Formula	C16H28N2O5Si
Molecular Weight	356.50 g/mol
Synonyms	5'-O-(tert-Butyldimethylsilyl)thymidine; 5'-O-TBDMS-Thymidine; 1-[(2R,3S,5R)-3-Hydroxy-5-[[(1,1-dimethylethyl)dimethylsilyl]oxy]tetrahydro-2-furanyl]-5-methyl-2,4(1H,3H)-pyrimidinedione; Thymidine 5'-O-tert-butyldimethylsilyl ether; TBDMS-Thymidine; 5'-O-(tert-Butyldimethylsilyl)-2'-deoxythymidine; 2'-Deoxythymidine 5'-(tert-butyldimethylsilyl) ether
EINECS	Contact for details

Quality Control

Our 5'-o-tert-Butyldimethylsilyl-Thymidine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for pharmaceutical research and development. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.