Description

3'-O-(T-Butyldimethylsilyl)Thymidine is a key protected nucleoside derivative, essential for the controlled synthesis of oligonucleotides and modified DNA strands. This compound is critical for researchers and manufacturers requiring high-purity intermediates to ensure the fidelity and yield of complex nucleic acid sequences. It is primarily utilized in pharmaceutical R&D, particularly in the development of antisense oligonucleotides, primers, and probes for diagnostic and therapeutic applications.

Application

• Oligonucleotide Synthesis: Serves as a crucial building block (phosphoramidite precursor) in solid-phase and solution-phase synthesis of DNA and RNA analogs.
• Antisense Therapy Development: Used in the research and production of antisense oligonucleotides targeting specific mRNA sequences for therapeutic intervention.
• Diagnostic Probe Manufacture: Employed in creating labeled probes for PCR, sequencing, and various hybridization assays.
• Pharmaceutical Intermediates: Acts as a protected intermediate in the multi-step synthesis of novel nucleoside-based antiviral or anticancer agents.
• Biochemical Research: Facilitates studies on DNA structure, function, and enzyme interactions by providing site-specifically modified nucleosides.
• Scale-Up GMP Production: Suitable for process development and cGMP manufacturing of active pharmaceutical ingredients (APIs) for clinical trials.

Basic Information

Product Name	3'-O-(T-Butyldimethylsilyl)Thymidine
CAS No.	40733-27-5
Molecular Formula	C16H28N2O6Si
Molecular Weight	372.50 g/mol
Synonyms	3'-O-TBDMS-Thymidine; 3'-O-(tert-Butyldimethylsilyl)thymidine; 1-[(2R,4S,5R)-4-[(tert-Butyldimethylsilyl)oxy]-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione; Thymidine 3'-O-tert-butyldimethylsilyl ether; 3'-TBDMS-Thd; 3'-O-(1,1-Dimethylethyl)dimethylsilyl]thymidine; 3'-O-[(1,1-Dimethylethyl)dimethylsilyl]-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl]-5-methyl-2,4(1H,3H)-pyrimidinedione
EINECS	Contact for details

Quality Control

Our 3'-O-(T-Butyldimethylsilyl)Thymidine is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and mass spectrometry, to ensure it meets the high standards required for pharmaceutical research and development. Certificates of Analysis (COA) with complete traceability are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Keep away from fluoride sources and strong alkali.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.