Description

Thymidine 5'-o-Pivaloate is a protected nucleoside derivative where the 5'-hydroxyl group is esterified with pivalic acid. This modification is crucial in oligonucleotide synthesis, serving as a key building block to prevent unwanted side reactions and ensure high coupling efficiency. It is an essential intermediate for researchers and manufacturers in the pharmaceutical and biotechnology sectors developing novel therapeutics, diagnostics, and genetic research tools.

Application

• Oligonucleotide Synthesis: A critical phosphoramidite precursor for the solid-phase synthesis of DNA and RNA strands.
• Antiviral & Anticancer Prodrug Development: Used in the research and synthesis of nucleoside analog prodrugs to enhance bioavailability and targeting.
• Bioconjugation & Labeling: Serves as a modified nucleoside for introducing specific functional groups or labels into nucleic acids.
• Medicinal Chemistry Research: A valuable intermediate for exploring structure-activity relationships in novel therapeutic compounds.
• Diagnostic Probe Manufacture: Employed in the production of labeled probes for techniques like FISH (Fluorescence In Situ Hybridization) and PCR.

Basic Information

Product Name	Thymidine 5'-o-Pivaloate
CAS No.	40733-25-3
Molecular Formula	C15H22N2O6
Molecular Weight	326.35 g/mol
Synonyms	5'-O-Pivaloylthymidine; 1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-methylpyrimidine-2,4-dione 5'-pivalate; Thymidine 5'-monopivalate; 2'-Deoxythymidine 5'-pivalate; 5'-O-(2,2-Dimethylpropanoyl)thymidine; Piv-dT
EINECS	Contact for details

Quality Control

Our Thymidine 5'-o-Pivaloate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and stringent controls for moisture and residual solvents. Certificates of Analysis (COA) detailing all specifications are provided to ensure compliance with your research and cGMP-grade manufacturing requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Related Substances (HPLC)	Total impurities ≤2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.