Description

5'-O-(4,4'-Dimethoxytrityl)-N2-Dimethylformamidine-2'-Deoxyguanosine is a highly specialized, protected nucleoside derivative essential for oligonucleotide synthesis. This compound serves as a critical phosphoramidite building block, enabling the precise and automated assembly of DNA sequences. It is primarily required by biotechnology, pharmaceutical, and diagnostic research sectors for applications in antisense therapy, PCR primer development, and genetic analysis.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA strands.
• Antisense and siRNA Therapeutic Development: For constructing modified oligonucleotides used in gene silencing and targeted drug discovery.
• PCR Primer and Probe Manufacturing: Enables the production of high-purity, sequence-specific primers and fluorescent probes for molecular diagnostics.
• Genomic Research and Next-Generation Sequencing (NGS): Used in the preparation of custom oligonucleotide libraries and sequencing adapters.
• Diagnostic Kit Production: A vital raw material for in-vitro diagnostic (IVD) assays, including those for infectious diseases and genetic disorders.
• Academic and Industrial R&D: Supports fundamental research in biochemistry, molecular biology, and synthetic biology.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N2-Dimethylformamidine-2'-Deoxyguanosine
CAS No.	40094-22-2
Molecular Formula	C35H39N7O6
Molecular Weight	653.73 g/mol
Synonyms	5'-O-DMT-N2-DMF-dG; DMT-DMF-dG Amidite Precursor; N2-(Dimethylaminomethylene)-2'-deoxy-5'-O-(4,4'-dimethoxytrityl)guanosine; 2'-Deoxy-5'-O-(4,4'-dimethoxytrityl)-N2-(dimethylaminomethylene)guanosine; Guanosine, 2'-deoxy-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N2-[(dimethylamino)methylene]-; Protected 2'-Deoxyguanosine Derivative; DMT-G(dmf) Monomer
EINECS	Contact for details

Quality Control

Our production of this high-purity building block adheres to stringent quality protocols suitable for pharmaceutical and diagnostic applications. Every batch is characterized using advanced analytical techniques including HPLC, NMR, and Mass Spectrometry to ensure structural identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and solvent residues is provided with each shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.