Description

3'-Fluorothymidine-5'-Triphosphate is a modified nucleotide triphosphate analog crucial for advanced molecular biology and diagnostic research. This compound matters as it serves as a key substrate for DNA polymerases, enabling the specific incorporation of a fluorinated thymidine analog into nucleic acid chains. Researchers in pharmaceutical development, molecular diagnostics, and biochemistry need it for applications such as probe synthesis, next-generation sequencing, and the study of enzyme kinetics and mechanisms.

Application

• Next-Generation Sequencing (NGS): Used as a specialized substrate for DNA polymerases in sequencing-by-synthesis platforms to study incorporation fidelity and develop novel sequencing chemistries.
• Molecular Probe Synthesis: Essential for enzymatically generating fluorinated DNA or RNA probes for fluorescence-based detection assays, including FISH (Fluorescence In Situ Hybridization).
• Enzyme Mechanism Studies: A critical tool for biochemists investigating the substrate specificity, kinetics, and inhibition of DNA polymerases, reverse transcriptases, and other nucleic acid-processing enzymes.
• Antiviral & Anticancer Research: Employed in the development and evaluation of nucleoside analog prodrugs, helping to understand their activation pathways and mechanisms of action.
• Diagnostic Assay Development: Integrated into the design of sensitive diagnostic kits that require the specific labeling or termination of DNA strands.
• Biophysical Labeling: Utilized to introduce a fluorine atom as a label for NMR-based structural studies of nucleic acids and protein-nucleic acid complexes.

Basic Information

Product Name	3'-Fluorothymidine-5'-Triphosphate
CAS No.	40026-13-9
Molecular Formula	C10H15FN2O13P3
Molecular Weight	498.13 g/mol
Synonyms	3'-F-dTTP; 3'-Fluoro-2'-deoxythymidine-5'-triphosphate; 3'-Fluoro-5'-TTP; 2'-Deoxy-3'-fluorothymidine-5'-triphosphate; FLT-TP; 3'-F-Thymidine triphosphate; Trifluridine triphosphate analog; 5'-Triphosphoric acid, 2'-deoxy-3'-fluorothymidine
EINECS	Contact for details

Quality Control

Our 3'-Fluorothymidine-5'-Triphosphate is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive research applications. Each batch is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, keep desiccated. Aliquot to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (HPLC)	≥ 90%
Water Content (KF)	≤ 5%
Solubility	Soluble in water, TE buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.