Description

3',5'-Bis-o-(Triphenylmethyl)Uridine is a protected nucleoside derivative where the 3' and 5' hydroxyl groups of the ribose sugar are masked with trityl (triphenylmethyl) protecting groups. This chemical modification is critical for controlling regioselectivity during oligonucleotide synthesis, particularly in the phosphoramidite method. It is an essential high-purity building block for researchers and manufacturers in the fields of therapeutic oligonucleotide development, antisense technology, and diagnostic probe synthesis.

Application

• Solid-Phase Oligonucleotide Synthesis: A key phosphoramidite precursor for the automated synthesis of RNA and DNA sequences.
• Antisense & siRNA Therapeutics: Used in the research and GMP-scale production of oligonucleotide-based drugs targeting genetic diseases.
• Diagnostic Probe Manufacture: Enables the creation of labeled probes for PCR, FISH, and microarray technologies.
• Academic & Industrial Research: Serves as a fundamental reagent for studying nucleic acid chemistry, structure, and function.
• Synthesis of Modified Nucleosides: An intermediate for introducing further modifications at the nucleoside level before oligomer assembly.

Basic Information

Product Name	3',5'-Bis-o-(Triphenylmethyl)Uridine
CAS No.	4710-75-2
Molecular Formula	C43H38N2O6
Molecular Weight	678.78 g/mol
Synonyms	3',5'-Di-O-trityluridine; 3',5'-Bis-O-(triphenylmethyl)uridine; Uridine, 3',5'-bis-O-(triphenylmethyl)-; 3',5'-Di-O-(triphenylmethyl)uridine; 3',5'-O-Ditrityluridine; 3',5'-Ditrityluridine; 2',3',5'-Tri-O-trityluridine (related isomer); 3',5'-Bis(trityl)uridine
EINECS	Contact for details

Quality Control

Our 3',5'-Bis-o-(Triphenylmethyl)Uridine is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive synthetic applications. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and impurity profiles. Quality is verified by advanced analytical techniques including HPLC, NMR, and mass spectrometry to meet the stringent requirements of pharmaceutical intermediate and research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent decomposition. For long-term storage, consider desiccant or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.