Description

3',5'-Di-o-(4-Methylbenzoyl)-Thymidine is a chemically modified nucleoside derivative, specifically a protected form of thymidine where the hydroxyl groups are esterified with p-toluoyl groups. This compound is a critical high-purity intermediate in the synthesis of oligonucleotides and modified nucleic acids, offering precise control over molecular structure and function. It is essential for pharmaceutical research and development, particularly in the fields of antisense therapy, diagnostics, and the creation of novel bioactive compounds.

Application

• Oligonucleotide Synthesis: A key phosphoramidite building block for the solid-phase synthesis of DNA and RNA strands in research and therapeutic development.
• Antisense Drug Development: Used in the production of antisense oligonucleotides (ASOs) designed to target specific RNA sequences for therapeutic intervention.
• Diagnostic Probe Manufacturing: Serves as a precursor for labeled nucleosides used in fluorescent probes, molecular beacons, and other diagnostic tools.
• Pharmaceutical Intermediate: An important raw material in the multi-step synthesis of complex nucleoside-based active pharmaceutical ingredients (APIs).
• Biochemical Research: Employed in academic and industrial labs to study nucleic acid chemistry, enzyme mechanisms, and to create custom-modified nucleotides.
• Prodrug Synthesis: Utilized in the design and synthesis of prodrugs where controlled release of the active nucleoside is required.

Basic Information

Product Name	3',5'-Di-o-(4-Methylbenzoyl)-Thymidine
CAS No.	4449-39-2
Molecular Formula	C26H26N2O8
Molecular Weight	494.49 g/mol
Synonyms	Thymidine, 3',5'-bis(4-methylbenzoate); 3',5'-Di-O-p-toluoylthymidine; 3',5'-Di-O-(p-toluoyl)thymidine; 1-[(2R,4S,5R)-4-[(4-Methylbenzoyl)oxy]-5-[[(4-methylbenzoyl)oxy]methyl]oxolan-2-yl]-5-methylpyrimidine-2,4-dione; 2'-Deoxythymidine 3',5'-bis(4-methylbenzoate); p-Toluoyl-protected thymidine
EINECS	Contact for details

Quality Control

Our 3',5'-Di-o-(4-Methylbenzoyl)-Thymidine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and Mass Spectrometry, to ensure it meets the high standards required for pharmaceutical research and synthesis. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.