Description

5'-Uridylic Acid, 2'-o-Methyl- is a chemically modified nucleotide monophosphate where a methyl group is introduced at the 2'-hydroxyl position of the ribose sugar. This specific modification is critical for enhancing the stability and biological activity of oligonucleotides, making it a valuable building block in advanced therapeutic and diagnostic platforms. It is primarily utilized by research institutions and biotechnology companies engaged in the development of antisense oligonucleotides, siRNA therapeutics, and diagnostic probes.

Application

• Antisense Oligonucleotide (ASO) Synthesis: A key phosphoramidite monomer for constructing oligonucleotides with improved nuclease resistance and binding affinity.
• siRNA and miRNA Research: Used to introduce stabilizing 2'-O-methyl modifications into RNA strands to enhance in vivo stability and efficacy.
• Diagnostic Probe Development: Incorporated into nucleic acid probes for assays requiring high specificity and stability, such as FISH or PCR.
• Structural Biology Studies: Employed to investigate RNA structure, function, and protein-RNA interactions.
• Therapeutic Aptamer Production: Serves as a modified nucleotide in creating aptamers with extended circulatory half-lives.
• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies involving nucleotidyltransferases and phosphorylases.

Basic Information

Product Name	5'-Uridylic Acid, 2'-o-Methyl-
CAS No.	4214-23-7
Molecular Formula	C10H15N2O9P
Molecular Weight	338.21 g/mol
Synonyms	2'-O-Methyluridine 5'-monophosphate; 2'-O-Methyl-UMP; Uridine, 2'-O-methyl-, 5'-(dihydrogen phosphate); 2'-O-Methyluridylic acid; 2'-OMe-UMP; pU2'OMe; 5'-UMP-2'-O-methyl
EINECS	Contact for details

Quality Control

Every batch of 5'-Uridylic Acid, 2'-o-Methyl- is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our quality system is designed to meet the stringent requirements for pharmaceutical intermediates and research-grade biochemicals. A comprehensive Certificate of Analysis (COA) detailing purity (typically by HPLC), identity confirmation (by NMR/IR/MS), and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccants and inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Related Substances (HPLC)	Total impurities ≤ 3.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.