Description

4Alpha-Methyl-5Alpha(H),14Alpha(H),17Alpha(H)-Cholestane is a high-purity, saturated sterane compound, widely recognized as a critical biomarker and reference standard in geochemical and environmental analysis. Its stable, fully hydrogenated structure makes it an invaluable internal standard for the quantification of sterols and other steroids in complex matrices. This compound is essential for researchers and quality control laboratories in the petroleum, environmental science, and analytical chemistry sectors who require precise calibration for chromatographic techniques like GC-MS.

Application

• Geochemical Biomarker Analysis: Serves as a key internal standard for the identification and quantification of steranes and diasteranes in crude oils and source rocks to assess thermal maturity and depositional environment.
• Environmental Forensics: Used as a reference compound in tracing the source and fate of organic pollutants, including sewage and petroleum hydrocarbons, in soil and water samples.
• Analytical Method Development: Employed in gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) methods for calibrating systems and validating analytical procedures for steroid analysis.
• Pharmaceutical Research: Acts as a stable, non-reactive analog in metabolic studies of cholesterol and related steroidal compounds.
• Quality Control Reference Standard: Provides a certified, high-purity material for the calibration of laboratory instruments and verification of analytical results in commercial testing labs.

Basic Information

Product Name	4Alpha-Methyl-5Alpha(H),14Alpha(H),17Alpha(H)-Cholestane
CAS No.	474-41-9
Molecular Formula	C28H50
Molecular Weight	386.70 g/mol
Synonyms	4α-Methyl-5α-cholestane; 4-Methylcholestane; 4α-Methylcholestane; 5α-Cholestane, 4α-methyl-; 4-Methyl-5α-cholestane; 4α-Methyl-5α,14α,17α-cholestane; NSC 92578
EINECS	207-472-5

Quality Control

Every batch of 4Alpha-Methyl-5Alpha(H),14Alpha(H),17Alpha(H)-Cholestane is manufactured and tested under strict quality management systems. We provide comprehensive analytical data to ensure the identity, purity, and consistency required for use as a reference standard. A detailed Certificate of Analysis (COA) is supplied with each shipment, documenting results from tests including identification, assay, and impurity profiling by chromatographic methods.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Keep the container tightly sealed in a dry environment to maintain long-term stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (GC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.