Description

3β,5,14-Trihydroxy-5β-Bufa-20,22-Dienolide is a high-purity bufadienolide compound, a class of cardioactive steroids. This specialized chemical is valued for its precise structure and biological activity, serving as a critical reference standard and intermediate in advanced research and development. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and manufacturers of fine chemicals for applications in cardiovascular studies and novel therapeutic agent synthesis.

Application

• Pharmaceutical Reference Standard: Used as a certified reference material (CRM) for the qualitative and quantitative analysis of bufadienolide-containing preparations in quality control laboratories.
• Biochemical Research: Serves as a key intermediate or probe in pharmacological studies investigating Na+/K+-ATPase inhibition and related cardiac effects.
• Fine Chemical Synthesis: Acts as a sophisticated building block for the semi-synthesis of novel steroid derivatives with potential therapeutic value.
• Academic Research: Employed in university and institutional labs for studies on the structure-activity relationships (SAR) of cardiac glycosides and bufadienolides.
• Analytical Method Development: Essential for developing and validating chromatographic (e.g., HPLC, LC-MS) methods for the detection of similar compounds in complex matrices.

Basic Information

Product Name	3β,5,14-Trihydroxy-5β-Bufa-20,22-Dienolide
CAS No.	472-26-4
Molecular Formula	C₂₄H₃₄O₅
Molecular Weight	402.52 g/mol
Synonyms	Telocinobufagin; 3β,5,14-Trihydroxy-19-oxo-5β,14β-bufa-20,22-dienolide; Bufa-20,22-dienolide, 3,5,14-trihydroxy-, (3β,5β)-; 19-Oxocinobufagin; NSC 107430; Cinobufagin aglycone; 3-Epicinobufagin
EINECS	Contact for details

Quality Control

Our 3β,5,14-Trihydroxy-5β-Bufa-20,22-Dienolide is produced under strict quality management protocols. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and development. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.