Description

3-Epibufalin is a specialized bufadienolide cardiac glycoside derivative, a compound of significant interest in advanced pharmaceutical research and development. Its unique stereochemistry at the 3-position makes it a critical intermediate and reference standard for studying structure-activity relationships in cardiotonic and potential anticancer agents. This high-purity compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and fine chemical sectors who require reliable, well-characterized building blocks for novel drug discovery and analytical method development.

Application

• Pharmaceutical Intermediate: Serves as a key chiral synthon in the synthesis of novel bufadienolide derivatives and analogs for cardiovascular and oncology research.
• Reference Standard: Used as a high-purity analytical standard in HPLC, LC-MS, and NMR for quality control and method validation in pharmaceutical manufacturing.
• Biochemical Research: Employed in pharmacological studies to investigate the mechanisms of Na+/K+-ATPase inhibition and its downstream effects on cell signaling pathways.
• Drug Discovery: A valuable scaffold for medicinal chemistry programs aimed at developing new cardiotonic agents with improved efficacy and safety profiles.
• Academic Research: Utilized in university and institutional labs for fundamental studies on natural product chemistry, biosynthesis, and semi-synthetic modification.

Basic Information

Product Name	3-Epibufalin
CAS No.	465-20-3
Molecular Formula	C24H34O5
Molecular Weight	402.53 g/mol
Synonyms	3-Epibufalin; 3-Epibufalin (3β-Hydroxy-14,15-epoxybufa-20,22-dienolide); 3-Epiresibufogenin; Bufa-20,22-dienolide, 3,14,15-trihydroxy-, (3β,5β,15β)-; 3β-Hydroxy-14,15-epoxybufa-20,22-dienolide; 3-Epibufogenin; NSC 83265
EINECS	Contact for details

Quality Control

Every batch of 3-Epibufalin CAS NO 465-20-3 is manufactured and analyzed under strict quality management systems. We employ advanced analytical techniques including HPLC, GC, NMR, and MS to ensure identity, purity, and consistency. A comprehensive Certificate of Analysis (COA) detailing purity, related substances, residual solvents, and other critical parameters is provided with each shipment. Our quality commitment aligns with cGMP principles for pharmaceutical intermediates, ensuring reliable performance in your most demanding applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Single Unknown Impurity	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.