Description

2' 5'-Dd-3'-Amp-Bis(T-Bu-Sate) is a specialized nucleoside phosphoramidite derivative, a critical building block in the automated synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of modified bases into DNA or RNA sequences, which is essential for advanced research and therapeutic development. This high-purity intermediate is indispensable for pharmaceutical R&D, diagnostic assay development, and academic research focused on antisense oligonucleotides, aptamers, and other nucleic acid-based technologies.

Application

• Oligonucleotide Synthesis: As a phosphoramidite monomer for the solid-phase synthesis of modified DNA/RNA strands.
• Therapeutic Development: Key raw material in the production of antisense oligonucleotides (ASOs) and siRNA for targeted therapies.
• Diagnostic Probes: Used to create labeled or modified probes for PCR, FISH, and microarray technologies requiring enhanced stability or specificity.
• Basic Research: Enables the study of nucleic acid structure, function, and protein interactions through site-specific modifications.
• Aptamer Production: Facilitates the generation of chemically modified aptamers with improved binding affinity and nuclease resistance.
• CRISPR/Cas System Enhancement: Potential use in synthesizing guide RNAs (gRNAs) with modified backbones for increased editing efficiency and delivery.

Basic Information

Product Name	2' 5'-Dd-3'-Amp-Bis(T-Bu-Sate)
CAS No.	387356-12-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-Deoxyadenosine-5'-O-DMT-3'-CED-phosphoramidite; dA Phosphoramidite (DMT); 2'-Deoxyadenosine Phosphoramidite; dA-CE Phosphoramidite; N6-Benzoyl-2'-deoxyadenosine-5'-O-(4,4'-dimethoxytrityl)-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite (common protected form); Adenine deoxyribonucleoside phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of 2' 5'-Dd-3'-Amp-Bis(T-Bu-Sate) is manufactured and tested under strict quality management systems. We guarantee high purity and consistency suitable for sensitive oligonucleotide synthesis applications. Our products undergo rigorous quality testing, including HPLC, NMR, and 31P NMR analysis, to ensure compliance with industry standards. Comprehensive Certificates of Analysis (COA) detailing purity, identity, and moisture content are provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at or below -20°C under an inert atmosphere (argon or nitrogen) due to its moisture-sensitive (hygroscopic) and potentially oxidizable nature. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture Content (KF)	≤ 1.0%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.