Description

Withanoside IV is a bioactive steroidal lactone glycoside, a key constituent derived from *Withania somnifera* (Ashwagandha). This high-purity reference standard is essential for research and development in pharmacology and natural product chemistry, offering a reliable benchmark for quality control and biological activity studies. It is primarily utilized by pharmaceutical R&D teams, nutraceutical manufacturers, and analytical laboratories focused on developing standardized herbal extracts and novel therapeutic agents.

Application

• Pharmaceutical Reference Standard: Serves as a certified primary standard for the identification, assay, and purity testing of Ashwagandha extracts and finished products.
• Bioactivity Research: Used in preclinical studies to investigate its neuroprotective, anti-inflammatory, anxiolytic, and adaptogenic properties.
• Nutraceutical Formulation: Employed in the standardization of high-quality dietary supplements to ensure consistent potency and efficacy.
• Analytical Method Development: Critical for developing and validating HPLC, LC-MS, and other chromatographic methods for phytochemical analysis.
• Quality Control (QC) & Assurance (QA): Provides an internal standard for in-process testing and final product release in GMP environments.
• Academic Research: Supports studies in phytochemistry, pharmacology, and biosynthetic pathway analysis.

Basic Information

Product Name	Withanoside IV
CAS No.	362472-81-9
Molecular Formula	C₄₀H₆₂O₁₄
Molecular Weight	766.91 g/mol
Synonyms	Withanoside IV; Withanoside 4; (20S,22R)-3β-[β-D-Glucopyranosyl-(1→6)-β-D-glucopyranosyloxy]-5α,20α(R)-dihydroxy-6α,7α-epoxy-1-oxowitha-2,24-dienolide; 27-Deoxywithanoside IV; Ashwagandha glycoside
EINECS	Contact for details

Quality Control

Every batch of Withanoside IV is manufactured and tested under strict quality management systems. Our products undergo rigorous identity, purity, and assay testing via advanced analytical techniques (HPLC, MS, NMR) to ensure compliance with research-grade and reference standard specifications. A comprehensive Certificate of Analysis (COA) detailing purity, chromatographic profile, and storage recommendations is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.