Description

n-(4-Azido-2-Nitrophenyl)-2-Aminoethylsulfonate, Sodium Salt, Dihydrate is a specialized photoactivatable crosslinking agent, widely recognized by its CAS number 352000-05-6. This compound is valued for its ability to form covalent bonds upon UV irradiation, making it an essential tool for irreversible biomolecule conjugation and surface modification. It is primarily used by researchers and manufacturers in the life sciences, diagnostics, and advanced materials sectors for applications requiring stable, photo-initiated linkages.

Application

• Bioconjugation and Protein Labeling: Used as a heterobifunctional crosslinker to attach proteins, peptides, or antibodies to surfaces, nanoparticles, or other biomolecules via its photoreactive azido group and amine-reactive sulfonate group.
• Surface Immobilization: Enables the covalent attachment of bioactive molecules to polymers, glass, or metal surfaces for creating biosensors, microarrays, and functionalized medical devices.
• Affinity Chromatography Ligand Coupling: Facilitates the stable immobilization of ligands onto chromatography resins for purification processes.
• Polymer and Hydrogel Modification: Incorporated into polymer backbones or used to functionalize hydrogels to introduce photoreactive sites for subsequent biomolecule grafting.
• Diagnostic Assay Development: Employed in the development of ELISA, lateral flow assays, and other diagnostic platforms for stable capture antibody immobilization.
• Materials Science Research: Serves as a key reagent in research focused on creating smart materials, self-assembled monolayers (SAMs), and patterned surfaces.

Basic Information

Product Name	n-(4-Azido-2-Nitrophenyl)-2-Aminoethylsulfonate, Sodium Salt, Dihydrate
CAS No.	352000-05-6
Molecular Formula	C8H8N5NaO6S · 2H2O
Molecular Weight	361.24 g/mol (Anhydrous basis)
Synonyms	ANPES, Sodium Salt; 2-[(4-Azido-2-nitrophenyl)amino]ethanesulfonic Acid Sodium Salt Dihydrate; 4-Azido-2-nitrophenyltaurine Sodium Salt; N-(4-Azido-2-nitrophenyl)taurine Sodium Salt; Photoactivatable Crosslinker ANPES; Sulfosuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate (Sulfo-SANPAH) related compound; NAP-Taurine, Sodium Salt; Aryl Azide Crosslinking Reagent.
EINECS	Contact for details

Quality Control

Our n-(4-Azido-2-Nitrophenyl)-2-Aminoethylsulfonate, Sodium Salt, Dihydrate is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure consistency and performance for critical research and manufacturing applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light will reduce its activity. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	8.0 - 11.0 % (Theoretical for dihydrate: ~9.97%)
Solubility	Soluble in water, DMSO; sparingly soluble in most organic solvents
UV Activity	Exhibits characteristic absorption spectrum (λmax ~260 nm & ~470 nm)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.