Description

N4-Benzoyl-2'-o-(2-Ethoxyethyl)-5-Methylcytidine is a key protected nucleoside derivative used as a sophisticated building block in oligonucleotide synthesis. This compound is specifically engineered to enhance the stability and solubility of nucleotide monomers during automated solid-phase synthesis processes. It is an essential raw material for research and production in the fields of therapeutic oligonucleotides, antisense drugs, and diagnostic probes, where precise chemical modification is critical for efficacy and stability.

Application

• Phosphoramidite precursor for the synthesis of modified oligonucleotides.
• Building block for antisense oligonucleotide (ASO) therapeutics targeting genetic diseases.
• Key intermediate in the research and development of small interfering RNA (siRNA) and microRNA (miRNA) therapeutics.
• Chemical modification for enhancing nuclease resistance and pharmacokinetic properties of oligonucleotide drugs.
• Production of diagnostic probes and primers requiring specific base modifications.
• Research tool in nucleic acid chemistry and medicinal chemistry for exploring structure-activity relationships.

Basic Information

Product Name	N4-Benzoyl-2'-o-(2-Ethoxyethyl)-5-Methylcytidine
CAS No.	340162-93-8
Molecular Formula	C22H29N3O7
Molecular Weight	447.48 g/mol
Synonyms	5-Methyl-2'-O-(2-ethoxyethyl)cytidine, N4-Benzoyl-5-methyl-2'-O-(2-ethoxyethyl)cytidine; 2'-O-(2-Ethoxyethyl)-5-methylcytidine, N4-benzoyl-; 5-Methyl-2'-O-(2-ethoxyethyl)-N4-benzoylcytidine; 2'-O-MOE-5-methylcytidine (N4-Benzoyl); Bz-5-Me-2'-MOE-C; Protected 5-Methylcytidine derivative; 2'-O-(2-Ethoxyethyl)-N4-benzoyl-5-methylcytidine
EINECS	Contact for details

Quality Control

Our N4-Benzoyl-2'-o-(2-Ethoxyethyl)-5-Methylcytidine is manufactured under strict quality systems suitable for pharmaceutical research and development. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure high chemical purity and structural identity. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a desiccated environment to prevent degradation. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.