Description

4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoyl Chloride is a specialized photoactivatable crosslinking reagent, a key building block in chemical biology and proteomics research. Its core value lies in the diazirine moiety, which generates highly reactive carbenes upon UV irradiation for irreversible covalent bonding with target biomolecules. This compound is essential for researchers and manufacturers developing photoaffinity probes, activity-based protein profiling (ABPP) reagents, and novel bioconjugation strategies in pharmaceutical discovery and life sciences.

Application

• Photoaffinity Labeling (PAL): Synthesis of probes for identifying and characterizing small molecule-protein interactions and binding sites.
• Chemical Proteomics: Development of activity-based probes (ABPs) for profiling enzyme function and activity in complex biological systems.
• Bioconjugation & Linker Chemistry: Serving as a reactive handle for introducing the photo-crosslinking diazirine group into peptides, proteins, or other molecular scaffolds.
• Material Science Surface Modification: Functionalizing surfaces or polymers with photoreactive groups for creating advanced bio-interfaces.
• Pharmaceutical Research: A critical intermediate in the discovery and development of covalent inhibitors and targeted therapies.
• Structural Biology: Aiding in the study of protein structures and dynamics through crosslinking mass spectrometry (XL-MS) techniques.

Basic Information

Product Name	4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoyl Chloride
CAS No.	326923-35-7
Molecular Formula	C10H4ClF3N2O
Molecular Weight	260.60 g/mol
Synonyms	3-(Trifluoromethyl)-3H-diazirin-3-yl]benzoyl chloride; 4-(3-(Trifluoromethyl)diazirin-3-yl)benzoyl chloride; 4-(3-(Trifluoromethyl)-3H-diazirin-3-yl)benzoic acid chloride; p-(3-(Trifluoromethyl)-3H-diazirin-3-yl)benzoyl chloride; TFAD Benzoyl Chloride; Photo-Leucine-Benzoyl Chloride analog; Diazirine-Benzoyl Chloride crosslinker
EINECS	Contact for details

Quality Control

Every batch of 4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]Benzoyl Chloride is synthesized and purified under controlled conditions to ensure high reactivity and consistency for sensitive research applications. Our products undergo rigorous quality testing, including HPLC, NMR, and MS analysis, to confirm identity, purity, and functional integrity. Certificates of Analysis (COA) with detailed chromatographic and spectroscopic data are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label or COA. This compound is hygroscopic (moisture-sensitive) and should be handled under an inert atmosphere (argon or nitrogen) when possible to maintain stability. Keep container tightly sealed in a desiccator for long-term storage.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.