Description

4-Isothiocyanato-2,2,6,6-Tetramethylpiperidine 1-Oxyl is a stable nitroxide radical functionalized with a reactive isothiocyanate group, serving as a critical spin label and molecular probe in advanced research. This compound is essential for site-directed spin labeling (SDSL) in electron paramagnetic resonance (EPR) spectroscopy, enabling the study of protein dynamics, structure, and conformational changes. It is primarily utilized by research institutions, pharmaceutical R&D departments, and specialty chemical manufacturers focused on biophysical chemistry and structural biology.

Application

• Site-Directed Spin Labeling (SDSL): Covalently attaches to cysteine residues in proteins for EPR spectroscopy studies of structure and dynamics.
• Protein Conformational Analysis: Acts as a molecular probe to monitor protein folding, unfolding, and interaction with ligands.
• Membrane Protein Studies: Used to label and investigate the structure and environment of integral membrane proteins.
• Polymer & Material Science: Serves as a radical initiator or a functional modifier in the synthesis of advanced polymers and smart materials.
• Nucleic Acid Research: Can be used to label modified nucleotides for studying nucleic acid structure and protein-nucleic acid interactions.
• Bioconjugation & Probe Synthesis: The reactive isothiocyanate group enables the creation of custom spin-labeled probes, antibodies, or other biomolecules.

Basic Information

Product Name	4-Isothiocyanato-2,2,6,6-Tetramethylpiperidine 1-Oxyl
CAS No.	36410-81-8
Molecular Formula	C10H17N2OS
Molecular Weight	213.32 g/mol
Synonyms	4-Isothiocyanato-TEMPO; TEMPO-Isothiocyanate; 2,2,6,6-Tetramethylpiperidine-1-oxyl-4-isothiocyanate; 4-Isothiocyanato-2,2,6,6-tetramethylpiperidin-1-oxyl; 4-NCS-TEMPO; Proxyl isothiocyanate; 1-Oxyl-2,2,6,6-tetramethyl-4-piperidyl isothiocyanate; 4-Isothiocyanato-TEMPO radical
EINECS	Contact for details

Quality Control

Our 4-Isothiocyanato-2,2,6,6-Tetramethylpiperidine 1-Oxyl is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, critical for sensitive research applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability parameters. We adhere to ISO 9001 quality management standards, and our processes are designed to meet the rigorous demands of analytical and research-grade chemical supply.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C under an inert atmosphere (e.g., nitrogen or argon) to minimize oxidation. Keep away from heat, sparks, and open flame. The container should be kept tightly sealed in a desiccator to protect from moisture.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
EPR Spectroscopy	Characteristic triplet signal
Melting Point	Contact for details
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.