Description

4α-Hydroxy Cholesterol is a specific hydroxylated derivative of cholesterol, a key intermediate in the study of steroid biosynthesis and metabolism. This compound matters for its role as a critical reference standard and biochemical tool in pharmaceutical research and development. It is primarily needed by researchers and manufacturers in the life sciences sector for applications in analytical chemistry, metabolic pathway studies, and as a precursor for synthesizing more complex steroid molecules.

Application

• Pharmaceutical Reference Standard: Used for the qualitative and quantitative analysis of sterols and related metabolites in drug development and quality control laboratories.
• Biochemical Research: Serves as a key intermediate or substrate in enzymatic studies related to cholesterol metabolism and steroid hormone biosynthesis pathways.
• Analytical Chemistry: Employed as a high-purity calibrant or internal standard in chromatographic methods like HPLC and GC-MS for accurate measurement of sterol profiles.
• Metabolite Studies: Utilized in metabolic and pharmacokinetic research to understand the oxidation products of cholesterol in biological systems.
• Academic & Institutional Research: A vital reagent for universities and research institutes conducting fundamental studies on lipid chemistry and cell biology.

Basic Information

Product Name	4α-Hydroxy Cholesterol
CAS No.	34310-86-6
Molecular Formula	C₂₇H₄₆O₂
Molecular Weight	402.66 g/mol
Synonyms	4α-Hydroxycholesterol; (3β)-Cholest-5-ene-3,4-diol; 4-Hydroxycholesterol (4α-isomer); 3β,4α-Dihydroxycholest-5-ene; 4α-Hydroxy-5-cholesten-3β-ol; NSC 163044; 4α-OHC
EINECS	Contact for details

Quality Control

Our 4α-Hydroxy Cholesterol is produced and handled under a strict quality management system. Each batch undergoes comprehensive analytical testing, including identity confirmation by spectroscopic methods and purity assessment by chromatography, to ensure it meets the high standards required for research applications. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.