Description

n-(Trimethylsilyl)-2'-O,3'-o-Bis(Trimethylsilyl)Adenosine 5'-[Phosphoric Acid Bis(Trimethylsilyl)] Ester is a highly protected adenosine derivative, extensively silylated to enhance stability and solubility in organic synthesis. This compound is a critical intermediate for the preparation of modified nucleotides and oligonucleotides, offering precise control over reactivity during complex coupling reactions. It is primarily utilized by research institutions and pharmaceutical companies engaged in nucleic acid chemistry, antisense drug development, and the synthesis of labeled probes for diagnostics.

Application

• Solid-Phase Oligonucleotide Synthesis: Acts as a key phosphoramidite precursor for the automated synthesis of DNA/RNA strands with specific modifications.
• Antisense and siRNA Therapeutic Development: Used in the research and production of therapeutic oligonucleotides targeting gene expression.
• Nucleotide Analog Synthesis: Serves as a protected building block for creating nucleoside triphosphate analogs used in enzymatic studies and sequencing.
• Diagnostic Probe Manufacturing: Enables the incorporation of reporter groups (e.g., fluorophores, biotin) into nucleic acid probes for assays and hybridization.
• Medicinal Chemistry Research: Facilitates the exploration of novel nucleoside-based drug candidates by providing a reactive, protected intermediate.
• Chemical Biology Tools: Used to synthesize cross-linking agents or affinity tags for studying nucleic acid-protein interactions.

Basic Information

Item	Detail
Product Name	n-(Trimethylsilyl)-2'-O,3'-o-Bis(Trimethylsilyl)Adenosine 5'-[Phosphoric Acid Bis(Trimethylsilyl)] Ester
CAS No.	32653-14-8
Molecular Formula	C27H59N5O7P Si5
Molecular Weight	770.27 g/mol
Synonyms	Adenosine, N-[trimethylsilyl]-2',3'-bis-O-(trimethylsilyl)-, 5'-(bis(trimethylsilyl) phosphate); 5'-O-[Bis(trimethylsilyl)phosphoryl]-N-(trimethylsilyl)-2',3'-O-bis(trimethylsilyl)adenosine; Protected Adenosine Phosphotriester; Silylated Adenosine Derivative; TMS-Adenosine Phosphotriester; CAS 32653-14-8
EINECS	Contact for details

Quality Control

Our production of this high-value synthetic intermediate adheres to stringent quality assurance protocols. Each batch is characterized using advanced analytical techniques including NMR, HPLC, and mass spectrometry to confirm identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment to ensure traceability and support your regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed under an inert atmosphere (argon or nitrogen) when not in use to prevent decomposition. Keep away from strong acids and bases.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.