Description

2'-Deoxy-n-Trimethylsilyl-3'-o-Trimethylsilylcytidine 5'-Phosphoric Acid Bis(Trimethylsilyl) Ester is a key protected nucleoside phosphoramidite derivative, essential for the automated synthesis of oligonucleotides. This compound matters because it provides the critical cytidine building block with protective groups that ensure high coupling efficiency and purity in DNA/RNA synthesis. It is primarily needed by pharmaceutical R&D teams, diagnostic developers, and biotechnology companies engaged in antisense therapy, PCR primer production, and genetic research.

Application

• Oligonucleotide Synthesis: Serves as a protected cytidine phosphoramidite monomer in solid-phase automated DNA/RNA synthesizers.
• Antisense & siRNA Therapeutics: A critical raw material for manufacturing therapeutic oligonucleotides targeting specific genes.
• PCR Primer & Probe Production: Used in the custom synthesis of primers and fluorescent probes for molecular diagnostics and research.
• Genetic Research Tools: Enables the synthesis of gene fragments, aptamers, and other modified nucleic acids for laboratory studies.
• Next-Generation Sequencing (NGS): Applied in the preparation of specialized oligonucleotide libraries and adapters.
• Diagnostic Assay Development: Integral to creating labeled probes for in-situ hybridization (ISH) and other detection platforms.

Basic Information

Product Name	2'-Deoxy-n-Trimethylsilyl-3'-o-Trimethylsilylcytidine 5'-Phosphoric Acid Bis(Trimethylsilyl) Ester
CAS No.	32645-72-0
Molecular Formula	C24H52N3O6PSi4
Molecular Weight	639.06 g/mol
Synonyms	5'-O-(Bis(trimethylsilyl)phosphoryl)-2'-deoxy-N,3'-O-bis(trimethylsilyl)cytidine; dC(Si) Phosphoramidite; N,3'-O-Bis(trimethylsilyl)-2'-deoxycytidine 5'-(bis(trimethylsilyl) phosphate); Protected Deoxycytidine Phosphoramidite; Cytidine, 2'-deoxy-N,3'-O-bis(trimethylsilyl)-, 5'-(bis(trimethylsilyl) phosphate); TMS-dC Phosphoramidite; CAS 32645-72-0
EINECS	Contact for details

Quality Control

Our 2'-Deoxy-n-Trimethylsilyl-3'-o-Trimethylsilylcytidine 5'-Phosphoric Acid Bis(Trimethylsilyl) Ester is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, IR). We provide comprehensive Certificates of Analysis (COA) detailing all specifications to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.