Description

3'-o-Trimethylsilylthymidine 5'-Phosphoric Acid Bis(Trimethylsilyl) Ester is a key protected nucleoside derivative used as a critical intermediate in oligonucleotide synthesis. This compound matters because it enables the precise and efficient construction of DNA sequences by protecting reactive hydroxyl and phosphate groups during automated solid-phase synthesis. It is primarily needed by manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors for producing primers, probes, and therapeutic oligonucleotides.

Application

• Oligonucleotide Synthesis: Serves as a protected thymidine phosphoramidite precursor for automated solid-phase DNA and RNA synthesis.
• Antisense & siRNA Therapeutics: A vital building block in the manufacturing of antisense oligonucleotides and small interfering RNA (siRNA) for therapeutic applications.
• Molecular Diagnostics: Used in the production of labeled probes and primers for PCR, sequencing, and genetic testing kits.
• Research & Development: Employed in academic and industrial labs for custom oligonucleotide synthesis and nucleic acid chemistry research.
• Bioconjugation: Acts as an intermediate for creating nucleotide conjugates with dyes, quenchers, or other functional groups.

Basic Information

Product Name	3'-o-Trimethylsilylthymidine 5'-Phosphoric Acid Bis(Trimethylsilyl) Ester
CAS No.	32645-60-6
Molecular Formula	C21H43N2O8PSi3
Molecular Weight	554.88 g/mol
Synonyms	Thymidine 3'-O-(trimethylsilyl) 5'-(bis(trimethylsilyl) phosphate); 5'-O-(Bis(trimethylsilyl)phosphoryl)-3'-O-trimethylsilylthymidine; 3'-O-TMS-thymidine 5'-(bis-TMS)phosphate; TMS-Thymidine Phosphotriester; 3'-O-Trimethylsilylthymidine 5'-(bis(trimethylsilyl) phosphate); Protected Thymidine Phosphotriester; Nucleoside Silyl Phosphate Ester
EINECS	Contact for details

Quality Control

Our 3'-o-Trimethylsilylthymidine 5'-Phosphoric Acid Bis(Trimethylsilyl) Ester is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, suitable for sensitive oligonucleotide synthesis. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP guidelines where applicable to support our customers' regulatory requirements for therapeutic and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions, preferably in a dry inert atmosphere, to maintain stability and prevent decomposition.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.