Description

3'-o-Methyladenosine-5'-Triphosphate Lithium Salt is a chemically modified nucleotide triphosphate analog, specifically methylated at the 3'-hydroxyl position of the ribose sugar. This structural modification makes it a valuable tool for studying and manipulating RNA metabolism, enzymatic mechanisms, and nucleotide-dependent processes. It is primarily utilized by researchers and developers in the fields of biochemistry, molecular biology, and pharmaceutical discovery. The compound is supplied as a high-purity lithium salt to ensure stability and solubility in aqueous research buffers.

Application

• Enzymology Research: Used as a substrate analog to investigate the specificity and kinetics of RNA polymerases, kinases, and other ATP-dependent enzymes.
• RNA Synthesis & Modification Studies: Employed in the study of RNA capping, termination, and processing mechanisms where a non-extendable nucleotide is required.
• Biochemical Assay Development: Serves as a critical component in high-throughput screening assays for drug discovery targeting nucleotide-binding proteins.
• Structural Biology: Aids in X-ray crystallography and NMR studies to elucidate the structure of enzyme active sites and their interactions with modified nucleotides.
• Diagnostic Reagent Manufacturing: Used in the formulation of specialized kits for molecular diagnostics and sequencing technologies.

Basic Information

Product Name	3'-o-Methyladenosine-5'-Triphosphate Lithium Salt
CAS No.	30993-48-7
Molecular Formula	C11H17N5O12P3·xLi+
Molecular Weight	Approx. 567.15 (Free acid) + xLi
Synonyms	3'-O-Methyl-ATP Lithium Salt; 3'-OMe-ATP Li Salt; 3'-Methyladenosine-5'-triphosphate Lithium; Adenosine-5'-triphosphate, 3'-O-methyl-, lithium salt; 3'-O-Methyl-ATP; 3'-Methoxy-ATP Lithium Salt; 3'-O-CH3-ATP Li+
EINECS	Contact for details

Quality Control

Our 3'-o-Methyladenosine-5'-Triphosphate Lithium Salt is manufactured under strict quality control protocols suitable for research and development applications. Each batch is analyzed to ensure high chemical purity and structural integrity. Certificates of Analysis (COA) are provided, detailing key parameters such as assay purity (HPLC), identity confirmation (NMR/IR), and levels of critical impurities like free nucleotides and lithium content. We adhere to in-house specifications designed to meet the rigorous demands of life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry atmosphere before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 95%
Purity (HPLC, Anion Exchange)	≥ 90%
Water Content (KF)	≤ 5.0%
pH (1% Solution)	6.0 - 8.0
Endotoxin (LAL)	< 10 EU/mg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.