Description

Adenosine 5'-(Tetrahydrogen Triphosphate), 2'-o-Methyl- is a chemically modified nucleotide analog where a methyl group replaces the hydroxyl at the 2' position of the ribose sugar. This modification confers significant resistance to enzymatic degradation, making it a critical reagent for studying RNA structure, function, and metabolism. It is primarily utilized by researchers and manufacturers in life science research, pharmaceutical development, and molecular diagnostics for applications requiring stable nucleotide incorporation.

Application

• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies, particularly for RNA polymerases, helicases, and other ATP-binding proteins.
• Antisense Oligonucleotide Synthesis: Serves as a building block (2'-O-methyl phosphoramidite) for the solid-phase synthesis of nuclease-resistant antisense and siRNA molecules.
• Structural Biology: Employed in X-ray crystallography and NMR studies to investigate protein-nucleotide interactions and RNA folding.
• Diagnostic Assay Development: Integrated into probes and primers for sensitive detection methods, enhancing stability against RNase activity.
• Pharmaceutical R&D: Investigated for potential therapeutic applications in antiviral and gene-silencing therapies.
• Enzyme Mechanism Studies: Acts as a non-hydrolyzable or slowly hydrolyzable ATP analog to probe kinetic mechanisms.

Basic Information

Product Name	Adenosine 5'-(Tetrahydrogen Triphosphate), 2'-o-Methyl-
CAS No.	30948-06-2
Molecular Formula	C11H18N5O12P3
Molecular Weight	541.20 g/mol
Synonyms	2'-O-Methyladenosine-5'-triphosphate; 2'-O-Methyl-ATP; 2'-OMe-ATP; Adenosine-5'-triphosphate, 2'-O-methyl-; 2'-Methyl ATP; 2'-O-Methyladenosine triphosphate; ATP, 2'-O-methyl-; 2'-OMe-adenosine triphosphate
EINECS	Contact for details

Quality Control

Our high-purity nucleotide analogs are manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure compliance with research-grade specifications. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay	≥ 90% (by UV spectrophotometry)
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.