Description

N3-Methyl-5-Methyluridine is a modified nucleoside derivative of uridine, featuring methylation at both the N3 and C5 positions. This specific structural modification is crucial for research in nucleic acid chemistry, impacting base pairing and stability. It serves as a valuable building block and reference standard for pharmaceutical and biotechnology researchers developing novel therapeutics, diagnostic probes, and studying RNA biology.

Application

• Pharmaceutical Intermediate: Key building block in the synthesis of novel nucleoside-based antiviral and anticancer agents.
• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies to understand RNA modification pathways and methyltransferase activity.
• Probe Development: Acts as a modified base for creating labeled nucleotides used in diagnostic assays and next-generation sequencing.
• Reference Standard: Serves as a high-purity analytical standard for quality control and method development in analytical laboratories.
• RNA Therapeutics: Investigated for incorporation into synthetic RNA (e.g., mRNA, siRNA) to modulate stability, immunogenicity, and translational efficiency.
• Chemical Biology Tool: Utilized to study the structure and function of RNA molecules through site-specific incorporation of methyl groups.

Basic Information

Product Name	N3-Methyl-5-Methyluridine
CAS No.	3650-91-7
Molecular Formula	C11H16N2O6
Molecular Weight	272.26 g/mol
Synonyms	3-Methyl-5-methyluridine; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)oxolan-2-yl)-3,5-dimethylpyrimidine-2,4-dione; 5-Methyl-3-methyluridine; 3,5-Dimethyluridine; N(3),5-Dimethyluridine; 2,4(1H,3H)-Pyrimidinedione, 1-β-D-ribofuranosyl-3,5-dimethyl-; 3,5-Dimethyl-1-β-D-ribofuranosyluracil
EINECS	Contact for details

Quality Control

Our N3-Methyl-5-Methyluridine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and mass spectrometry, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.