Description

N(4)-Hydroxycytidine is a modified nucleoside derivative of cytidine, characterized by a hydroxyl group substitution at the N4 position. This structural modification is of significant interest in pharmaceutical research and development, particularly in the study of nucleoside analogs and their biological activities. It serves as a key intermediate or reference standard for researchers in the fields of antiviral drug discovery, oncology, and molecular biology. The compound is supplied with a high level of purity to ensure reliable and reproducible results in sensitive applications.

Application

• Pharmaceutical Intermediate: Critical building block in the synthesis of novel nucleoside-based antiviral and anticancer agents.
• Biochemical Research: Used as a reference standard or substrate in enzymatic studies, metabolic pathway analysis, and molecular biology experiments.
• Antiviral Drug Development: Investigated for its potential role as a metabolite or active component in the development of broad-spectrum antiviral therapies.
• Oncology Research: Studied for its incorporation into nucleic acids and its effects on cancer cell proliferation and apoptosis.
• Academic & Contract Research: Essential reagent for universities and CROs (Contract Research Organizations) conducting fundamental research in medicinal chemistry and biochemistry.

Basic Information

Product Name	N(4)-Hydroxycytidine
CAS No.	3258-02-4
Molecular Formula	C9H13N3O6
Molecular Weight	259.22 g/mol
Synonyms	4-Hydroxycytidine; N4-Hydroxycytidine; 1-β-D-Ribofuranosyl-4-hydroxy-2(1H)-pyrimidinone; 4-Hydroxy-1-β-D-ribofuranosyl-2(1H)-pyrimidinone; Hydroxycytidine; Cytidine, N4-hydroxy-; Iso-cytidine
EINECS	221-874-6

Quality Control

Our N(4)-Hydroxycytidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and tests for residual solvents and moisture content. We provide a Certificate of Analysis (COA) with every shipment, detailing all specifications and test results to ensure traceability and compliance with your research or development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container should be kept tightly sealed after each use to prevent degradation from atmospheric moisture. For long-term storage, consider desiccants or inert atmosphere conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.