Description

Methacholine Bromide is a synthetic cholinergic agonist and a quaternary ammonium compound, widely recognized for its potent muscarinic receptor activity. This high-purity chemical is essential for research and diagnostic applications, particularly in pulmonary function testing. It is a critical reagent for pharmaceutical research, clinical diagnostics, and the development of autonomic nervous system studies. Global suppliers and research institutions rely on consistent quality and reliable supply of this specialized compound.

Application

• Bronchial Challenge Testing: Primarily used as the active pharmaceutical ingredient (API) in solutions for methacholine challenge tests (MCT) to diagnose and assess airway hyperresponsiveness in conditions like asthma.
• Pharmacological Research: Serves as a standard muscarinic acetylcholine receptor (mAChR) agonist in preclinical studies to investigate cholinergic pathways, receptor function, and signal transduction.
• Clinical Diagnostics Manufacturing: Incorporated into standardized diagnostic kits and solutions used in pulmonary function laboratories and clinical settings worldwide.
• Autonomic Nervous System Studies: Utilized in experimental models to study parasympathetic stimulation, smooth muscle contraction, and cardiovascular responses.
• Reference Standard: Employed as a high-purity analytical reference standard in quality control (QC) and method development for HPLC, titration, and other analytical techniques.
• Academic & Educational Use: Applied in university and institutional laboratories for teaching principles of pharmacology, respiratory physiology, and receptor-ligand interactions.

Basic Information

Product Name	Methacholine Bromide
CAS No.	333-31-3
Molecular Formula	C8H18BrNO2
Molecular Weight	240.14 g/mol
Synonyms	Acetyl-β-methylcholine bromide; (2-Hydroxypropyl)trimethylammonium bromide acetate; Mecholyl Bromide; β-Methylacetylcholine bromide; Provocholine® (brand name); AHR-968; Methacholine Bromide Salt; (2-Hydroxypropyl)trimethylammonium acetate bromide
EINECS	206-356-7

Quality Control

Our Methacholine Bromide is manufactured and tested under a strict quality management system. Each batch is analyzed to meet high-purity standards suitable for research and diagnostic applications. Comprehensive testing includes identification, assay, impurity profiling, and residual solvent analysis. A Certificate of Analysis (COA) detailing all test results and methods (e.g., HPLC, IR) is provided with every shipment to ensure full traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed after opening to prevent degradation from atmospheric moisture. Keep away from incompatible materials.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥ 98.0% (on anhydrous basis)
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.