Description

Evans Blue is a high-purity, water-soluble diazo dye widely recognized for its vital role in biomedical research and diagnostics. Its core value lies in its unique property of binding to serum albumin, making it an indispensable tool for assessing vascular permeability and blood volume. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic reagent sectors. We supply Evans Blue CAS NO 314-13-6 to the global market with a commitment to consistent quality and reliable supply chain logistics.

Application

• Vascular Permeability Studies: Primarily used as a tracer to quantify and visualize the leakage of plasma proteins from blood vessels in animal models of inflammation, sepsis, and cancer.
• Blood Volume Determination: Employed in physiological and pharmacological research to measure total circulating blood volume in laboratory animals.
• Blood-Brain Barrier (BBB) Integrity Assessment: A standard marker for evaluating the disruption or integrity of the BBB in neurological disease research.
• Diagnostic Reagent Component: Used in the formulation of specific in vitro diagnostic kits and laboratory stains.
• Protein Binding Studies: Serves as a model compound for studying the binding characteristics of drugs to serum albumin.
• Histological Staining: Applied in specialized tissue staining protocols to highlight vascular structures.

Basic Information

Item	Detail
Product Name	Evans Blue
CAS No.	314-13-6
Molecular Formula	C34H24N6Na4O14S4
Molecular Weight	960.81 g/mol
Synonyms	Evans Blue; Direct Blue 53; C.I. 23860; Azovan Blue; T-1824; Diamine Sky Blue; Pontamine Sky Blue; 3,3'-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(2,1-diazenediyl)]bis[5-amino-4-hydroxy-2,7-naphthalenedisulfonic acid] tetrasodium salt
EINECS	206-242-5

Quality Control

Every batch of Evans Blue is manufactured and tested under a strict quality management system. We provide material that meets high-purity standards suitable for research and in vitro diagnostic use. Comprehensive testing includes identity confirmation, purity assay, and analysis of key impurities. A Certificate of Analysis (COA) detailing all test results is provided with each shipment to ensure full traceability and compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive (store away from light). Keep the container tightly sealed to protect from moisture.

Specification

Item	Specification
Appearance	Blue to blue-green powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 5.0 %
Absorbance Ratio (A610/A740)	1.20 - 1.40
pH (1% Solution)	5.0 - 7.0
Water Solubility	Completely soluble

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.