Description

Novobiocin is a naturally occurring aminocoumarin antibiotic produced by *Streptomyces niveus*. This compound is a potent inhibitor of bacterial DNA gyrase, specifically targeting the GyrB subunit, which makes it a valuable tool in biochemical and microbiological research. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and manufacturers of diagnostic reagents for studying antibiotic resistance mechanisms, bacterial protein function, and as a selective agent in cell culture. Our supply of Novobiocin CAS NO 303-81-1 is characterized by high purity and reliable batch-to-batch consistency to support critical research and development work.

Application

• Biochemical Research: Used as a specific inhibitor of DNA gyrase (GyrB) to study bacterial DNA replication, transcription, and repair mechanisms.
• Microbiological Studies: Employed in antimicrobial susceptibility testing and for the selection and maintenance of bacterial strains in genetic studies.
• Pharmaceutical R&D: Serves as a reference standard and active pharmaceutical ingredient (API) in the development of novel antibacterial agents.
• Cell Culture: Acts as a selective agent in mammalian cell culture media to prevent bacterial contamination, particularly in sensitive bioprocessing.
• Diagnostic Manufacturing: Incorporated into culture media and kits used for the identification and differentiation of bacterial pathogens in clinical diagnostics.
• Veterinary Medicine: Historically used in topical formulations for the treatment of mastitis in cattle, with ongoing research into new applications.

Basic Information

Product Name	Novobiocin
CAS No.	303-81-1
Molecular Formula	C31H36N2O11
Molecular Weight	612.63 g/mol
Synonyms	Albamycin; Cathomycin; Cardelmycin; Inamycin; Vulcamycin; Streptonicate; 7-[[4-O-(Aminocarbonyl)-2,6-dideoxy-β-L-lyxo-hexopyranosyl]oxy]-4-hydroxy-3-(4-hydroxy-3-(3-methylbut-2-en-1-yl)benzamido)-8-methylcoumarin-2-carboxylic acid
EINECS	206-147-9

Quality Control

Our Novobiocin is manufactured and tested under a strict quality management system. Each batch is analyzed to ensure it meets high-purity standards suitable for research and development applications. A comprehensive Certificate of Analysis (COA) is provided with every shipment, detailing key parameters such as identity, purity, and impurity profiles. We can support compliance requirements for various pharmacopeial standards (e.g., USP) upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to pale yellow crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Specific Rotation	Contact for details
Solubility	Soluble in DMSO, methanol; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.