Description

N3-Ethyl Pseudouridine is a chemically modified nucleoside analog of pseudouridine, a critical component in the development of advanced mRNA therapeutics and vaccines. This specific modification at the N3 position enhances the stability and translational efficiency of synthetic mRNA, addressing key challenges in therapeutic efficacy and immunogenicity. It is an essential raw material for pharmaceutical R&D and manufacturing, particularly for companies focused on next-generation genetic medicines, vaccine platforms, and oligonucleotide synthesis.

Application

• mRNA Therapeutic & Vaccine Manufacturing: A key building block for synthesizing modified mRNA to improve protein expression and reduce innate immune recognition.
• Oligonucleotide Synthesis: Used in the solid-phase synthesis of modified RNA strands for research and therapeutic applications.
• Biochemical Research: A vital tool for studying RNA structure, function, and the impact of specific nucleoside modifications on biological systems.
• Antiviral Drug Development: Investigated as a component in nucleoside analog prodrugs targeting viral polymerases.
• Diagnostic Probe Development: Incorporated into labeled probes for detecting specific RNA sequences with enhanced stability.
• Cell & Gene Therapy: Supports the production of mRNA used in ex vivo cell engineering and in vivo gene editing platforms like CRISPR.

Basic Information

Product Name	N3-Ethyl Pseudouridine
CAS No.	2413585-37-0
Molecular Formula	C11H16N2O6
Molecular Weight	272.26 g/mol
Synonyms	1-Ethylpseudouridine; 3-Ethylpseudouridine; N3-Ethyl-5-β-D-ribofuranosyluracil; Pseudouridine, 3-ethyl-; 3-Ethyl-5-ribosyluracil; 5-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-3-ethylpyrimidine-2,4(1H,3H)-dione
EINECS	Contact for details

Quality Control

Our N3-Ethyl Pseudouridine is manufactured under strict quality management systems. Each batch is subjected to rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture to ensure it meets the stringent requirements for pharmaceutical and research applications. Certificates of Analysis (COA) with full traceability are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This material is hygroscopic (moisture-sensitive) and should be handled under dry, inert conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.