Description

Thalidomide-o-Peg4-Azide is a specialized bifunctional linker molecule designed for advanced bioconjugation and PROTAC (Proteolysis Targeting Chimera) development. This compound integrates the well-characterized thalidomide moiety, which binds to cereblon (CRBN), with a tetraethylene glycol (PEG4) spacer and a terminal azide group for copper-free click chemistry. It is engineered to enable the precise construction of targeted protein degraders and facilitates the modular assembly of complex chemical probes for drug discovery and chemical biology research.

Application

• PROTAC & Molecular Glue Development: Serves as a critical warhead component for designing novel CRBN-recruiting degraders targeting proteins of interest (POIs).
• Chemical Biology Probes: Used in the synthesis of activity-based probes for studying E3 ubiquitin ligase function and protein-protein interactions.
• ADC (Antibody-Drug Conjugate) Linker Exploration: Functions as a cleavable linker candidate, connecting cytotoxic payloads to antibodies via bioorthogonal chemistry.
• Bioconjugation & Labeling: The terminal azide enables efficient, site-specific conjugation to alkyne-modified biomolecules (e.g., proteins, oligonucleotides) for labeling and immobilization.
• Materials Science & Surface Functionalization: Applied to modify surfaces and nanomaterials with bioactive molecules for biosensing and diagnostic applications.
• Library Synthesis for Drug Discovery: A key building block for generating diverse compound libraries focused on targeted protein degradation pathways.

Basic Information

Product Name	Thalidomide-o-Peg4-Azide
CAS No.	2380318-57-8
Molecular Formula	C27H35N5O10
Molecular Weight	589.60 g/mol
Synonyms	Thalidomide-PEG4-azide; (S)-Thalidomide-O-PEG4-N3; 2-(2,6-Dioxopiperidin-3-yl)-4-((2-(2-(2-(2-azidoethoxy)ethoxy)ethoxy)ethoxy)methyl)isoindoline-1,3-dione; CRBN Ligand-PEG4-Azide; Thalidomide-O-tetraethylene glycol-azide; E3 Ligase Ligand-Linker Conjugate 1; PROTAC Linker for CRBN; N-(Azido-PEG4)-thalidomide
EINECS	Contact for details

Quality Control

Our Thalidomide-o-Peg4-Azide is manufactured under strict quality systems. Each batch undergoes comprehensive analytical characterization, including HPLC for purity assessment, NMR (1H, 13C) and HRMS for structural confirmation, and chiral analysis to ensure enantiomeric purity. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee compliance with research-grade specifications and support reproducible results in your applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. Store away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Chiral Purity (HPLC)	≥ 99% ee

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.