Description

Azido-PEG12-NHS ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester at one terminus and a versatile azide group at the other. This compound is essential for creating stable, site-specific conjugations between biomolecules and surfaces or other molecules via bioorthogonal click chemistry. It is primarily utilized by researchers and developers in the fields of bioconjugation, pharmaceutical development, and materials science for applications such as antibody-drug conjugate (ADC) synthesis, peptide modification, and surface functionalization.

Application

• Bioconjugation and ADC Development: Site-specific conjugation of drugs, dyes, or other payloads to antibodies, proteins, or peptides via the azide-alkyne cycloaddition (CuAAC or SPAAC) reaction.
• PEGylation Reagent: Introduction of a long, hydrophilic PEG12 spacer to enhance the solubility, stability, and pharmacokinetic properties of therapeutic biomolecules.
• Surface Functionalization: Immobilization of biomolecules onto azide- or alkyne-modified surfaces, nanoparticles, or chips for biosensor and diagnostic assay development.
• Polymer and Materials Science: A key building block for synthesizing functionalized hydrogels, smart polymers, and drug delivery systems through click chemistry.
• Chemical Biology Probes: Creation of activity-based probes for labeling and studying biological targets in complex cellular environments.
• Diagnostic Reagent Synthesis: Development of conjugated reagents for immunoassays, fluorescence imaging, and other diagnostic platforms.

Basic Information

Product Name	Azido-PEG12-NHS Ester
CAS No.	2363756-50-5
Molecular Formula	C33H60N4O16
Molecular Weight	768.85 g/mol
Synonyms	NHS-PEG12-Azide; Azido-PEG12-NHS ester; N3-PEG12-NHS; 1-Azido-3,6,9,12,15,18,21,24,27,30,33,36-dodecaoxaoctatetracontan-38-oic acid 2,5-dioxopyrrolidin-1-yl ester; PEG12-Azide-NHS Ester; Azido-dodecaethylene glycol-NHS ester; N-(Azido-PEG12)-N-hydroxysuccinimide ester
EINECS	Contact for details

Quality Control

Our Azido-PEG12-NHS ester is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is characterized by NMR, HPLC, and mass spectrometry to confirm identity, purity, and functional integrity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (Azide)	≥ 90%
Functional Group Assay (NHS Ester)	≥ 90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.