Description

3'-o-Methyl-5-Methyluridine is a modified nucleoside analog of significant interest in advanced pharmaceutical and biochemical research. This compound is valued for its role as a key building block and intermediate in the synthesis of oligonucleotides, including modified RNA and DNA strands. It is primarily sought by researchers and manufacturers in the fields of nucleic acid therapeutics, antisense technology, and pro-drug development. Its specific methylation pattern offers unique properties for tuning the stability, binding affinity, and pharmacokinetic profiles of novel therapeutic agents.

Application

• Pharmaceutical Intermediate: Critical building block for the synthesis of novel nucleoside-based antiviral and anticancer agents.
• Oligonucleotide Synthesis: Used in solid-phase and solution-phase synthesis to incorporate specific modifications into RNA and DNA sequences.
• Antisense & siRNA Research: Serves as a modified monomer to enhance the nuclease resistance and target affinity of therapeutic oligonucleotides.
• Biochemical Probe Development: Utilized in creating labeled or tagged nucleotides for studying nucleic acid metabolism and enzyme mechanisms.
• Pro-drug Synthesis: Acts as a precursor in the design of masked nucleoside analogs for improved cellular delivery and activation.
• Academic & Contract Research: Essential reagent for universities and CROs exploring nucleic acid chemistry and biology.

Basic Information

Product Name	3'-o-Methyl-5-Methyluridine
CAS No.	2305415-87-4
Molecular Formula	C12H16N2O6
Molecular Weight	284.26 g/mol
Synonyms	5-Methyl-3'-O-methyluridine; 3'-O-Methyl-5-methyluridine; 1-((2R,3R,4R,5R)-3-Fluoro-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione (related isomer); 5-Methyl-2'-deoxy-3'-O-methyluridine (common misnomer); Uridine, 3'-O-methyl-5-methyl-; Modified Nucleoside Monomer; RNA Modification Intermediate
EINECS	Contact for details

Quality Control

Our 3'-o-Methyl-5-Methyluridine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via NMR and MS. We provide full traceability and detailed Certificates of Analysis (COA) upon request, ensuring the material meets the stringent requirements for research and cGMP-grade pharmaceutical synthesis.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.