Description

5-(Azidomethyl)-2'-o-(2-Methoxyethyl)Uridine is a modified nucleoside building block of significant interest in advanced pharmaceutical and biotechnology research. This compound is specifically engineered for applications in oligonucleotide synthesis, particularly for the development of therapeutic antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs). It is a critical raw material for research institutions, CROs/CDMOs, and pharmaceutical companies engaged in the discovery and manufacturing of next-generation nucleic acid-based therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A key phosphoramidite precursor for the automated synthesis of modified oligonucleotides.
• Antisense Oligonucleotide (ASO) Therapeutics: Used to incorporate specific modifications that enhance stability, target affinity, and pharmacokinetic properties of therapeutic ASOs.
• siRNA and miRNA Mimics/Antagonists: Essential for constructing chemically modified siRNA strands to improve nuclease resistance and reduce off-target effects.
• Diagnostic Probe Development: Serves as a modified base in the synthesis of fluorescent or affinity-labeled nucleic acid probes for diagnostic assays.
• Xenonucleic Acid (XNA) Research: A valuable monomer for exploring the properties and applications of synthetic nucleic acid analogs with altered sugar-phosphate backbones.
• Click Chemistry Conjugation: The azidomethyl group enables efficient, site-specific bioconjugation via copper-catalyzed azide-alkyne cycloaddition (CuAAC) for attaching reporter molecules or functional groups.

Basic Information

Product Name	5-(Azidomethyl)-2'-o-(2-Methoxyethyl)Uridine
CAS No.	2305415-77-2
Molecular Formula	C13H19N5O7
Molecular Weight	357.32 g/mol
Synonyms	2'-O-Methoxyethyl-5-azidomethyluridine; 5-(Azidomethyl)-2'-O-(2-methoxyethyl)uridine; 2'-MOE-5-azidomethyluridine; Uridine, 5-(azidomethyl)-2'-O-(2-methoxyethyl)-; 5-N3Me-2'-MOE-U; 2'-O-(2-Methoxyethyl)-5-(azidomethyl)uridine; 5-Azidomethyl-2'-MOE-U
EINECS	Contact for details

Quality Control

Our 5-(Azidomethyl)-2'-o-(2-Methoxyethyl)Uridine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research and GMP-grade applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to stringent in-process controls and final release testing protocols, including advanced chromatographic and spectroscopic methods, to meet the exacting standards of the oligonucleotide therapeutics industry.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.