Description

Azidoethyl-Ss-Propionic NHS Ester is a heterobifunctional crosslinking reagent featuring a reactive NHS ester and an azido group. This compound is specifically engineered for the efficient and selective conjugation of biomolecules, enabling the formation of stable amide bonds with primary amines. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, antibody-drug conjugate (ADC) development, and proteomics who require reliable, high-purity linkers for their critical workflows.

Application

• Bioconjugation and Protein Labeling: Used to attach fluorescent dyes, peptides, or other probes to proteins and antibodies via amine groups.
• Antibody-Drug Conjugate (ADC) Synthesis: Serves as a key linker component for attaching cytotoxic payloads to monoclonal antibodies.
• Surface Functionalization: Enables the modification of amine-coated surfaces, nanoparticles, or microarrays with azide groups for subsequent click chemistry reactions.
• Polymer and Material Science: Facilitates the crosslinking and functionalization of amine-containing polymers to introduce bio-orthogonal azide handles.
• Proteomics Research: Employed in activity-based protein profiling (ABPP) and other chemical proteomics strategies to tag and enrich target proteins.
• Diagnostic Reagent Development: Used in the creation of sensitive immunoassay components and diagnostic conjugates.

Basic Information

Product Name	Azidoethyl-Ss-Propionic NHS Ester
CAS No.	2243566-44-9
Molecular Formula	C₁₀H₁₄N₄O₅
Molecular Weight	270.24 g/mol
Synonyms	3-(2-Azidoethoxy)propanoic acid N-hydroxysuccinimide ester; 2,5-Dioxopyrrolidin-1-yl 3-(2-azidoethoxy)propanoate; Azido-PEG2-NHS ester; NHS-PEG2-Azide; N-(3-(2-Azidoethoxy)propanoyloxy)succinimide; Azidoethyl-SS-Propionate NHS Ester; Azido-PEG2-propionate NHS ester; Azido-PEG2-C2-NHS ester
EINECS	Contact for details

Quality Control

Our Azidoethyl-Ss-Propionic NHS Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and FTIR for functional group verification, to ensure it meets the high standards required for sensitive bioconjugation applications. A detailed Certificate of Analysis (COA) is provided with every shipment, guaranteeing traceability, consistency, and reliability for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.