Description

3'-o-(2-Methoxyethyl)-5-Methylcytidine is a modified nucleoside building block of significant interest in advanced pharmaceutical research and development. This compound is a key intermediate in the synthesis of oligonucleotides, particularly for therapeutic applications requiring enhanced stability and pharmacokinetic properties. It is primarily utilized by research institutions, biotechnology firms, and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNA, and other novel nucleic acid-based therapeutics.

Application

• Oligonucleotide Synthesis: A critical phosphoramidite precursor for the solid-phase synthesis of therapeutic oligonucleotides.
• Antisense Drug Development: Used in the manufacture of antisense oligonucleotides (ASOs) designed to target specific RNA sequences.
• siRNA Therapeutics: Serves as a modified monomer to enhance the nuclease resistance and efficacy of small interfering RNA (siRNA) constructs.
• Research & Development: Employed in academic and industrial labs for studying structure-activity relationships (SAR) of nucleic acid analogs.
• Diagnostic Probe Development: Can be incorporated into nucleic acid probes for diagnostic assays requiring high specificity and stability.
• Pharmaceutical Intermediates: Acts as a high-value intermediate in the multi-step synthesis of active pharmaceutical ingredients (APIs) for genetic medicines.

Basic Information

Product Name	3'-o-(2-Methoxyethyl)-5-Methylcytidine
CAS No.	2243311-50-2
Molecular Formula	C13H21N3O6
Molecular Weight	315.32 g/mol
Synonyms	5-Methylcytidine, 3'-O-(2-methoxyethyl)-; 3'-O-Methoxyethyl-5-methylcytidine; 3'-O-(2-Methoxyethyl)-5-methylcytidine; 5-Methyl-3'-O-(2-methoxyethyl)cytidine; 3'-O-(2-Methoxyethyl)-5-methylcytidine (MOE-5-Me-C); 3'-O-MOE-5-methylcytidine; 5-Methylcytidine 3'-(2-methoxyethyl) ether; 3'-O-(β-Methoxyethyl)-5-methylcytidine
EINECS	Contact for details

Quality Control

Our 3'-o-(2-Methoxyethyl)-5-Methylcytidine is manufactured under strict quality systems suitable for research and pharmaceutical intermediate applications. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets stringent specifications. Certificates of Analysis (COA) documenting purity, related substances, and residual solvents are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Individual impurity ≤ 0.5%
Total Impurities	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.