Description

3'-o-Azidomethyl-7-Tfa-Ap-7-Deaza-Da is a specialized nucleoside phosphoramidite derivative, a critical building block in oligonucleotide synthesis. Its value lies in enabling the precise incorporation of modified 7-deaza-adenosine analogs, which are essential for enhancing the stability and performance of synthetic oligonucleotides. This high-purity intermediate is primarily needed by research institutions and pharmaceutical companies engaged in the development of advanced therapeutics, including antisense oligonucleotides, aptamers, and diagnostic probes.

Application

• Oligonucleotide Synthesis: As a phosphoramidite monomer for the solid-phase synthesis of DNA/RNA strands containing modified nucleobases.
• Therapeutic Oligonucleotide R&D: Key raw material for producing antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs) with improved nuclease resistance and binding affinity.
• Aptamer Development: Used in creating chemically modified aptamers for targeted drug delivery and diagnostic applications.
• Probe and Primer Design: Enables the synthesis of fluorescently labeled or otherwise tagged probes and primers for advanced genomic research and diagnostics.
• Structural Biology Studies: Facilitates the study of nucleic acid structure and function by incorporating stable analogs.
• Cross-linking and Bioconjugation: The azidomethyl group provides a handle for "click chemistry" reactions, allowing for site-specific conjugation with other molecules.

Basic Information

Product Name	3'-o-Azidomethyl-7-Tfa-Ap-7-Deaza-Da
CAS No.	2232127-48-7
Molecular Formula	C31H38N9O7P
Molecular Weight	679.68 g/mol
Synonyms	1H-Pyrrolo[2,3-d]pyrimidin-4-amine, 7-[(2R,3R,4R,5R)-5-[bis(1-methylethyl)amino](2-cyanoethoxy)phosphinooxy]-4-[[(1,1-dimethylethyl)dimethylsilyl]oxy]-2-[[(trifluoroacetyl)amino]methyl]-3-[(2-azidoacetyl)oxy]tetrahydro-2-furanyl]-2-fluoro-; 7-Deaza-2'-deoxy-7-iodoadenosine-5'-triphosphate; 7-Deaza-dA Phosphoramidite (Azidomethyl protected); N-Trifluoroacetyl-3'-O-azidomethyl-2'-deoxy-7-deazaadenosine-5'-phosphoramidite; 3'-O-(Azidomethyl)-N6-trifluoroacetyl-2'-deoxy-7-deazaadenosine-5'-phosphoramidite; 7-Deaza-2'-deoxyadenosine derivative phosphoramidite.
EINECS	Contact for details

Quality Control

Our 3'-o-Azidomethyl-7-Tfa-Ap-7-Deaza-Da is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC for purity and NMR for structural confirmation. Certificates of Analysis (COA) detailing all specifications are provided to guarantee the material meets the stringent requirements for pharmaceutical R&D and production.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C ± 5°C under an inert atmosphere (argon or nitrogen). The product is hygroscopic (moisture-sensitive) and must be handled in a dry environment to maintain stability and reactivity.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.