Description

N1-Ethoxymethyl Pseudouridine is a chemically modified nucleoside derivative, specifically engineered for advanced oligonucleotide synthesis. This compound is critical for enhancing the stability, efficacy, and pharmacokinetic properties of therapeutic nucleic acids, such as mRNA and siRNA. It is an essential raw material for pharmaceutical R&D and manufacturing, particularly for companies developing next-generation vaccines, gene therapies, and RNA-based therapeutics.

Application

• mRNA Vaccine & Therapeutic Manufacturing: A key modified building block for synthesizing mRNA to improve translational efficiency and reduce immunogenicity.
• siRNA and Antisense Oligonucleotide Production: Used to incorporate stability-enhancing modifications into RNA strands for targeted gene silencing applications.
• Pharmaceutical Research & Development: Serves as a critical reagent in the discovery and optimization of novel RNA-based drug candidates.
• Diagnostic Probe Synthesis: Employed in the creation of high-stability, sensitive nucleic acid probes for molecular diagnostics and bioanalytical assays.
• Academic & Biotech Research: A valuable tool for studying RNA structure, function, and metabolism in basic and applied life sciences.

Basic Information

Product Name	N1-Ethoxymethyl Pseudouridine
CAS No.	2131744-33-5
Molecular Formula	C12H18N2O7
Molecular Weight	302.28 g/mol
Synonyms	1-(Ethoxymethyl)pseudouridine; 5-[(2S,3R,4S,5R)-3,4-Dihydroxy-5-[(ethoxymethyl)amino]oxolan-2-yl]-1H-pyrimidine-2,4-dione; N1-EM Pseudouridine; Pseudouridine, 1-(ethoxymethyl)-; Ethoxymethyl Pseudouridine; Modified Nucleoside for mRNA; RNA Modification Reagent
EINECS	Contact for details

Quality Control

Our N1-Ethoxymethyl Pseudouridine is manufactured under strict quality management systems to meet the exacting standards of pharmaceutical and biotech applications. Each batch is subjected to comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and stringent controls for residual solvents and impurities. Certificates of Analysis (COA) detailing all specifications are provided with every shipment to ensure full traceability and compliance with cGMP guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled under a dry inert atmosphere or in a controlled environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.