Description

3'-F-3'-Da(Bz)-2'-Phosphoramidite is a specialized, high-purity phosphoramidite monomer, a critical building block in the automated solid-phase synthesis of oligonucleotides. This compound is engineered to introduce a 3'-fluoro modification, which is essential for enhancing the nuclease resistance and binding affinity of therapeutic nucleic acids. It is primarily required by biotechnology and pharmaceutical companies engaged in the research and development of antisense oligonucleotides, siRNA, and other advanced genetic medicines.

Application

• Synthesis of Fluorinated Oligonucleotides: Key monomer for incorporating 3'-fluoro-deoxyadenosine analogs into DNA/RNA sequences.
• Antisense Drug Development: Used in creating oligonucleotides with improved metabolic stability for therapeutic applications.
• siRNA and miRNA Research: Essential for producing modified siRNA strands to optimize gene silencing efficacy and pharmacokinetic profiles.
• Diagnostic Probe Manufacture: Employed in the synthesis of high-specificity fluorescent or radioactive probes for genetic testing.
• Academic & Industrial R&D: A vital reagent for studying structure-activity relationships (SAR) in nucleic acid chemistry.
• GMP-grade Production: Suitable for scaling up to support clinical and commercial manufacturing of oligonucleotide-based therapeutics.

Basic Information

Product Name	3'-F-3'-Da(Bz)-2'-Phosphoramidite
CAS No.	2127174-09-6
Molecular Formula	C41H49FN5O7P
Molecular Weight	789.84 g/mol
Synonyms	3'-Fluoro-3'-deoxyadenosine, N6-Benzoyl-2'-deoxy-2'-fluoro-adenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-Deoxy-2'-fluoro-N6-benzoyladenosine-3'-CE phosphoramidite; 3'-F-dA(Bz) Phosphoramidite; 3'-Fluoro-2'-deoxyadenosine phosphoramidite, N6-Benzoyl protected; 5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-2'-fluoro-N6-benzoyladenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
EINECS	Contact for details

Quality Control

Our 3'-F-3'-Da(Bz)-2'-Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC for purity and 31P/1H/19F NMR for structural confirmation. Certificates of Analysis (COA) with detailed specifications are provided and can be tailored to meet specific GMP or research-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer. This material is hygroscopic (moisture-sensitive) and should be handled in a controlled, dry environment. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.