Description

2'-o-Methyl-5-Methyl-4-Thiouridine is a chemically modified nucleoside analog, a key building block in advanced oligonucleotide synthesis. This compound is critical for researchers and manufacturers developing next-generation therapeutic and diagnostic agents, particularly in the fields of antisense oligonucleotides and RNA-based technologies. It is primarily utilized by pharmaceutical R&D teams, biotechnology companies, and fine chemical suppliers focused on nucleic acid chemistry and medicinal chemistry applications.

Application

• Oligonucleotide Synthesis: A vital phosphoramidite precursor for the solid-phase synthesis of modified RNA and DNA strands.
• Antisense Therapy Development: Used to create oligonucleotides with enhanced nuclease resistance and improved binding affinity for target mRNA sequences.
• RNAi Research: Incorporated into small interfering RNA (siRNA) and microRNA (miRNA) constructs to study gene silencing mechanisms and improve stability.
• Diagnostic Probe Design: Serves as a modified base in fluorescent or quencher-labeled probes for real-time PCR and other molecular detection assays.
• Medicinal Chemistry: Employed in structure-activity relationship (SAR) studies to optimize the pharmacokinetic properties of nucleic acid-based drug candidates.
• Bioconjugation: Acts as a handle for site-specific labeling or attachment of other functional groups to oligonucleotides.

Basic Information

Product Name	2'-o-Methyl-5-Methyl-4-Thiouridine
CAS No.	2095417-26-6
Molecular Formula	C11H16N2O5S
Molecular Weight	288.32 g/mol
Synonyms	4-Thio-2'-O-methyl-5-methyluridine; 5-Methyl-2'-O-methyl-4-thiouridine; 2'-O-Methyl-5-methyl-4-thiouridine; 4-Thiouridine, 2'-O-methyl-5-methyl-; Modified Nucleoside 4-Thio Uridine Derivative; RNA Building Block 2095417-26-6
EINECS	Contact for details

Quality Control

Our 2'-o-Methyl-5-Methyl-4-Thiouridine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and MS, to ensure it meets the high standards required for pharmaceutical research and oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.